Immunostimulatory properties of heat-resistant RNA in a decoction of Glycyrrhizae Radix.
Animals
Drugs, Chinese Herbal
/ pharmacology
Glycyrrhiza
/ genetics
Glycyrrhizic Acid
/ pharmacology
High-Throughput Nucleotide Sequencing
/ methods
Macrophages
/ metabolism
Mice
NF-kappa B
/ metabolism
Phytochemicals
/ genetics
Plant Extracts
/ pharmacology
Plants, Medicinal
/ chemistry
Tumor Necrosis Factor-alpha
/ metabolism
Glycyrrhizae radix
Nuclear Factor-κB (NF-κB)
RNA
RNA-Sequencing
Tumor Necrosis Factor-α (TNF-α)
Journal
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie
ISSN: 1950-6007
Titre abrégé: Biomed Pharmacother
Pays: France
ID NLM: 8213295
Informations de publication
Date de publication:
Jan 2022
Jan 2022
Historique:
received:
02
09
2021
revised:
19
11
2021
accepted:
19
11
2021
pubmed:
6
12
2021
medline:
11
3
2022
entrez:
5
12
2021
Statut:
ppublish
Résumé
Research on the bioactive components of herbal medicines have been conducted mainly on the secondary metabolites of herbal plants. Accordingly, limited information is available on primary metabolites (carbohydrates, amino acids, lipids, and nucleic acids) and their biological effects. Here, we focused on the heat-resistant RNA of a decoction of Glycyrrhizae Radix and showed its immunostimulatory effects. The RNA activated NF-κB/AP-1 and induced TNF-α production in murine macrophages. Further analysis revealed that the RNA was around 90 nucleotides long. RNA sequencing (RNA-Seq) by next generation sequencing (NGS) showed that approximately 30% of the NGS reads were mapped to the genome of Glycyrrhiza uralensis, which is plant material of Glycyrrhizae Radix. Further analysis of the other 70% of reads indicated that the RNA contained RNA sequences that could be mapped to various microorganisms. Together, these results propose nucleic acids as a new research field in the bioactive components of herbal medicines.
Identifiants
pubmed: 34864611
pii: S0753-3322(21)01243-9
doi: 10.1016/j.biopha.2021.112457
pii:
doi:
Substances chimiques
Drugs, Chinese Herbal
0
NF-kappa B
0
Phytochemicals
0
Plant Extracts
0
Tumor Necrosis Factor-alpha
0
Glycyrrhizic Acid
6FO62043WK
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
112457Informations de copyright
Copyright © 2021 The Authors. Published by Elsevier Masson SAS.. All rights reserved.