Multimodal Spectroscopic Imaging of Pea Root Nodules to Assess the Nitrogen Fixation in the Presence of Biofertilizer Based on Nod-Factors.
Fertilizers
/ microbiology
Leghemoglobin
/ metabolism
Nitrogen Fixation
/ physiology
Pisum sativum
/ growth & development
Rhizobium leguminosarum
/ metabolism
Root Nodules, Plant
/ metabolism
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Spectroscopy, Fourier Transform Infrared
Spectrum Analysis, Raman
FT-IR spectroscopy
MALDI MSI
Nod factor
Raman spectroscopy
Rhizobium
pea
symbiosis
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
30 Nov 2021
30 Nov 2021
Historique:
received:
10
11
2021
revised:
28
11
2021
accepted:
29
11
2021
entrez:
10
12
2021
pubmed:
11
12
2021
medline:
21
12
2021
Statut:
epublish
Résumé
Multimodal spectroscopic imaging methods such as Matrix Assisted Laser Desorption/Ionization Mass Spectrometry Imaging (MALDI MSI), Fourier Transform Infrared spectroscopy (FT-IR) and Raman spectroscopy were used to monitor the changes in distribution and to determine semi quantitatively selected metabolites involved in nitrogen fixation in pea root nodules. These approaches were used to evaluate the effectiveness of nitrogen fixation by pea plants treated with biofertilizer preparations containing Nod factors. To assess the effectiveness of biofertilizer, the fresh and dry masses of plants were determined. The biofertilizer was shown to be effective in enhancing the growth of the pea plants. In case of metabolic changes, the biofertilizer caused a change in the apparent distribution of the leghaemoglobin from the edges of the nodule to its centre (the active zone of nodule). Moreover, the enhanced nitrogen fixation and presumably the accelerated maturation form of the nodules were observed with the use of a biofertilizer.
Identifiants
pubmed: 34884793
pii: ijms222312991
doi: 10.3390/ijms222312991
pmc: PMC8657664
pii:
doi:
Substances chimiques
Fertilizers
0
Leghemoglobin
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : the National Centre for Research and Development within the Lider VIII programme
ID : LIDER/11/0070/L-8/16/NCBR/2017
Organisme : Ministry of Science and Higher Education in Poland
ID : DS42/2021 project.
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