Liquid-state NMR spectroscopy for complex carbohydrate structural analysis: A hitchhiker's guide.

Carbohydrate Conformation Carbohydrates / analysis Magnetic Resonance Spectroscopy
Carbohydrates Chemical shifts analysis Glycans NMR Spectra interpretation Spectra processing

Journal

Carbohydrate polymers
ISSN: 1879-1344
Titre abrégé: Carbohydr Polym
Pays: England
ID NLM: 8307156

Informations de publication

Date de publication:
01 Feb 2022
Historique:
received: 29 08 2021
revised: 23 10 2021
accepted: 09 11 2021
entrez: 11 12 2021
pubmed: 12 12 2021
medline: 17 3 2022
Statut: ppublish

Résumé

Structural determination of carbohydrates is mostly performed by liquid-state NMR, and it is a demanding task because the NMR signals of these biomolecules explore a rather narrow range of chemical shifts, with the result that the resonances of each monosaccharide unit heavily overlap with those of others, thus muddling their punctual identification. However, the full attribution of the NMR chemical shifts brings great advantages: it discloses the nature of the constituents, the way they are interconnected, in some cases their absolute configuration, and it paves the way to other and more sophisticated analyses. The purpose of this review is to provide a practical guide into this challenging subject. It will drive through the strategy used to assign the NMR data, pinpointing the core information disclosed from each NMR experiment, and suggesting useful tricks for their interpretation, along with other resources pivotal during the study of these biomolecules.

Identifiants

DOI: 10.1016/j.carbpol.2021.118885 PMID: 34893288
pubmed: 34893288
pii: S0144-8617(21)01272-8
doi: 10.1016/j.carbpol.2021.118885
pii:
doi:

Substances chimiques

Carbohydrates 0

Types de publication

Journal Article Review

Langues

eng

Sous-ensembles de citation

IM

Pagination

118885

Informations de copyright

Copyright © 2021. Published by Elsevier Ltd.

Auteurs

Immacolata Speciale (I)

Department of Agricultural Sciences, University of Naples, 80055 Portici, Italy. Electronic address: immacolata.speciale@unina.it.

Anna Notaro (A)

Department of Agricultural Sciences, University of Naples, 80055 Portici, Italy. Electronic address: anna.notaro@unina.it.

Pilar Garcia-Vello (P)

Department of Chemical Sciences, University of Naples, 80126 Naples, Italy. Electronic address: pilargvello@gmail.com.

Flaviana Di Lorenzo (F)

Department of Agricultural Sciences, University of Naples, 80055 Portici, Italy. Electronic address: flaviana.dilorenzo@unina.it.

Samantha Armiento (S)

Department of Chemical Sciences, University of Naples, 80126 Naples, Italy. Electronic address: samantha.armiento@unina.it.

Antonio Molinaro (A)

Department of Chemical Sciences, University of Naples, 80126 Naples, Italy. Electronic address: molinaro@unina.it.

Roberta Marchetti (R)

Department of Chemical Sciences, University of Naples, 80126 Naples, Italy. Electronic address: roberta.marchetti@unina.it.

Alba Silipo (A)

Department of Chemical Sciences, University of Naples, 80126 Naples, Italy. Electronic address: alba.silipo@unina.it.

Cristina De Castro (C)

Department of Agricultural Sciences, University of Naples, 80055 Portici, Italy. Electronic address: decastro@unina.it.

Articles similaires

Drug repurposing against fucosyltransferase-2 via docking, STD-NMR, and molecular dynamic simulation studies.

Muhammad Atif, Humaira Zafar, Atia-Tul- Wahab et al.
1.00
Fucosyltransferases Drug Repositioning Molecular Docking Simulation Molecular Dynamics Simulation Humans

Nitrile glove composition and performance-Substandard properties and inaccurate packaging information.

Ashley Herkins, Sriloy Dey, Dan Conroy et al.
1.00
Nitriles Tensile Strength Materials Testing Gloves, Protective Product Packaging

The conformational landscape of fold-switcher KaiB is tuned to the circadian rhythm timescale.

Hannah K Wayment-Steele, Renee Otten, Warintra Pitsawong et al.
1.00
Rhodobacter sphaeroides Bacterial Proteins Circadian Rhythm Protein Folding Protein Conformation

Development of a

Julian Mevenkamp, Yvonne M H Bruls, Rodrigo Mancilla et al.
1.00
Humans NAD Magnetic Resonance Spectroscopy Male Muscle, Skeletal

Classifications MeSH

questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Conformation moléculaire › Conformation des glucides › Liquid-state NMR spectroscopy for complex...
questionsmedicales.fr › Glucides › Liquid-state NMR spectroscopy for complex...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Analyse spectrale › Spectroscopie par résonance magnétique › Liquid-state NMR spectroscopy for complex...