A System for In Vitro Generation of Mature Murine Plasma Cells Uncovers Differential
Journal
Journal of immunology (Baltimore, Md. : 1950)
ISSN: 1550-6606
Titre abrégé: J Immunol
Pays: United States
ID NLM: 2985117R
Informations de publication
Date de publication:
15 01 2022
15 01 2022
Historique:
received:
07
01
2021
accepted:
29
10
2021
pubmed:
17
12
2021
medline:
2
2
2022
entrez:
16
12
2021
Statut:
ppublish
Résumé
Upon encounter with Ag, B cells undergo a sequential process of differentiation to become Ab-secreting plasma cells. Although the key drivers of differentiation have been identified, research has been limited by the lack of in vitro models recapitulating the full process for murine B cells. In this study, we describe methodology using BCR or TLR ligation to obtain plasma cells that are phenotypically mature, have exited cell cycle and express a gene signature concordant with long-lived plasma cells. Dependent on the initial stimuli, the transcriptomes also show variation including the enhanced expression of matrisome components after BCR stimulation, suggestive of unique functional properties for the resultant plasma cells. Moreover, using the new culture conditions we demonstrate that alternative promoter choice regulating the expression of the master transcription factor Blimp-1/Prdm1 can be observed; when the canonical B cell promoter for
Identifiants
pubmed: 34911772
pii: jimmunol.2100004
doi: 10.4049/jimmunol.2100004
pmc: PMC7612223
mid: EMS138082
doi:
Substances chimiques
Prdm1 protein, mouse
0
Receptors, Antigen, B-Cell
0
Positive Regulatory Domain I-Binding Factor 1
EC 2.1.1.-
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
514-525Subventions
Organisme : Cancer Research UK
ID : A17723
Pays : United Kingdom
Organisme : Cancer Research UK
ID : A19349
Pays : United Kingdom
Informations de copyright
Copyright © 2022 The Authors.
Références
Mol Cell Biol. 2009 Nov;29(21):5813-27
pubmed: 19737919
Biostatistics. 2007 Jan;8(1):118-27
pubmed: 16632515
J Immunol. 2006 Oct 1;177(7):4584-93
pubmed: 16982896
J Immunol. 2012 Dec 15;189(12):5773-85
pubmed: 23162129
Bioinformatics. 2019 Jun 1;35(12):2084-2092
pubmed: 30395178
Nat Rev Immunol. 2015 Mar;15(3):160-71
pubmed: 25698678
Immunol Rev. 2020 Jul;296(1):87-103
pubmed: 32592168
BMC Bioinformatics. 2011 Aug 04;12:323
pubmed: 21816040
Leukemia. 2014 Aug;28(8):1647-56
pubmed: 24504026
J Exp Med. 1994 Jul 1;180(1):157-63
pubmed: 7516405
Nat Immunol. 2016 Mar;17(3):323-30
pubmed: 26779600
Nat Immunol. 2004 Mar;5(3):299-308
pubmed: 14985713
Nat Immunol. 2015 Jun;16(6):663-73
pubmed: 25894659
Nucleic Acids Res. 2000 Dec 15;28(24):4846-55
pubmed: 11121475
Cell Immunol. 1991 Jun;135(1):95-104
pubmed: 1902147
Nat Commun. 2018 Sep 12;9(1):3698
pubmed: 30209264
J Immunol. 2004 Nov 1;173(9):5556-63
pubmed: 15494505
Nat Immunol. 2016 Mar;17(3):331-43
pubmed: 26779602
Genome Biol. 2014;15(12):550
pubmed: 25516281
J Allergy Clin Immunol. 2009 Jun;123(6):1277-86.e5
pubmed: 19427685
J Immunol. 1992 Jun 15;148(12):3830-6
pubmed: 1602131
Mol Cell Biol. 2012 Sep;32(17):3403-13
pubmed: 22733990
J Immunol. 2010 Nov 15;185(10):6058-67
pubmed: 20944005
Nat Immunol. 2004 Jan;5(1):55-63
pubmed: 14647274
Reproduction. 2008 Oct;136(4):503-14
pubmed: 18583473
Autoimmunity. 2015 Feb;48(1):1-12
pubmed: 25536171
J Immunol. 1986 Dec 15;137(12):3855-60
pubmed: 2878044
J Exp Med. 1978 Feb 1;147(2):297-315
pubmed: 564390
Nat Commun. 2018 Apr 27;9(1):1698
pubmed: 29703886
F1000Res. 2015 Dec 30;4:1521
pubmed: 26925227
J Exp Med. 1994 Nov 1;180(5):1889-900
pubmed: 7964465
Mol Cell Biol. 2000 Apr;20(7):2592-603
pubmed: 10713181
Blood. 2009 Dec 10;114(25):5173-81
pubmed: 19846886
Eur J Immunol. 2001 Jul;31(7):1972-80
pubmed: 11449349
J Immunol. 2010 Sep 15;185(6):3117-25
pubmed: 20814029
Bioinformatics. 2013 Jan 1;29(1):15-21
pubmed: 23104886
NPJ Syst Biol Appl. 2019 Apr 11;5:13
pubmed: 30993001
J Immunol. 1999 Oct 15;163(8):4175-81
pubmed: 10510353
Cytometry A. 2014 Jan;85(1):15-24
pubmed: 24700574
Genome Res. 2012 Oct;22(10):2008-17
pubmed: 22722343
Immunity. 2003 Oct;19(4):607-20
pubmed: 14563324
Cold Spring Harb Perspect Biol. 2012 Jan 01;4(1):a004903
pubmed: 21937732
Immunity. 1994 Jun;1(3):167-78
pubmed: 7534202
Immunol Rev. 2010 Sep;237(1):140-59
pubmed: 20727034
J Exp Med. 2014 Sep 22;211(10):2103-18
pubmed: 25180063
Front Immunol. 2018 Sep 10;9:2053
pubmed: 30250473