Generation of three human induced pluripotent stem cell lines with IRX5 knockout and knockin genetic editions using CRISPR-Cas9 system.

Animals CRISPR-Cas Systems / genetics Heterozygote Homeodomain Proteins / genetics Homozygote Humans Induced Pluripotent Stem Cells / metabolism Myocytes, Cardiac / metabolism Transcription Factors / genetics

Journal

Stem cell research
ISSN: 1876-7753
Titre abrégé: Stem Cell Res
Pays: England
ID NLM: 101316957

Informations de publication

Date de publication:
01 2022
Historique:
received: 26 11 2021
accepted: 12 12 2021
pubmed: 21 12 2021
medline: 16 4 2022
entrez: 20 12 2021
Statut: ppublish

Résumé

Studies on animal models have shown that Irx5 is an important regulator of cardiac development and that it regulates ventricular electrical repolarization gradient in the adult heart. Mutations in IRX5 have also been linked in humans to cardiac conduction defects. In order to fully characterize the role of IRX5 during cardiac development and in cardiomyocyte function, we generated three genetically-modified human induced pluripotent stem cell lines: two knockout lines (heterozygous and homozygous) and a knockin HA-tagged line (homozygous).

Identifiants

DOI: 10.1016/j.scr.2021.102627 PMID: 34929443
pubmed: 34929443
pii: S1873-5061(21)00474-8
doi: 10.1016/j.scr.2021.102627
pii:
doi:

Substances chimiques

Homeodomain Proteins 0
IRX5 protein, human 0
Transcription Factors 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

102627

Informations de copyright

Copyright © 2021. Published by Elsevier B.V.

Auteurs

Robin Canac (R)

Université de Nantes, CHU Nantes, CNRS, INSERM, l'institut du thorax, F-44000 Nantes, France.

Amandine Caillaud (A)

Université de Nantes, CHU Nantes, CNRS, INSERM, l'institut du thorax, F-44000 Nantes, France.

Bastien Cimarosti (B)

Université de Nantes, CHU Nantes, CNRS, INSERM, l'institut du thorax, F-44000 Nantes, France.

Aurore Girardeau (A)

Université de Nantes, CHU Nantes, CNRS, INSERM, l'institut du thorax, F-44000 Nantes, France.

Hanan Hamamy (H)

Department of Genetic Medicine and Development, Geneva University, Geneva 1211, Switzerland.

Bruno Reversade (B)

Genome Institute of Singapore, A*STAR, Singapore, Singapore; Medical Genetics Department, Koç University School of Medicine (KUSOM), Istanbul, Turkey; Department of Paediatrics, National, University of Singapore, Singapore, Singapore; Institute of Molecular and Cell Biology, A*STAR, Singapore, Singapore; Reproductive Biology Laboratory, Amsterdam UMC, Amsterdam-Zuidoost, the Netherlands.

Carine Bonnard (C)

Skin Research Institute of Singapore, A*STAR, Singapore, Singapore.

Zeina R Al Sayed (ZR)

Université de Nantes, CHU Nantes, CNRS, INSERM, l'institut du thorax, F-44000 Nantes, France.

Laurent David (L)

Université de Nantes, CHU Nantes, INSERM, CNRS, SFR Santé, FED 4203, INSERM UMS 016, CNRS, UMS 3556, F-44000 Nantes, France; Université de Nantes, CHU Nantes, INSERM, CRTI, UMR 1064, ITUN, F-44000 Nantes, France.

Jeremie Poschmann (J)

Université de Nantes, CHU Nantes, INSERM, CRTI, UMR 1064, ITUN, F-44000 Nantes, France.

Patricia Lemarchand (P)

Université de Nantes, CHU Nantes, CNRS, INSERM, l'institut du thorax, F-44000 Nantes, France.

Guillaume Lamirault (G)

Université de Nantes, CHU Nantes, CNRS, INSERM, l'institut du thorax, F-44000 Nantes, France.

Nathalie Gaborit (N)

Université de Nantes, CHU Nantes, CNRS, INSERM, l'institut du thorax, F-44000 Nantes, France. Electronic address: nathalie.gaborit@univ-nantes.fr.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Generation of three human induced pluripotent...
questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Épigenèse génétique › Extinction de l'expression des gènes › Systèmes CRISPR-Cas › Generation of three human induced pluripotent...
questionsmedicales.fr › Phénomènes génétiques › Génotype › Hétérozygote › Generation of three human induced pluripotent...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines de liaison à l'ADN › Protéines à homéodomaine › Generation of three human induced pluripotent...
questionsmedicales.fr › Phénomènes génétiques › Génotype › Homozygote › Generation of three human induced pluripotent...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Generation of three human induced pluripotent...
questionsmedicales.fr › Cellules › Cellules souches › Cellules souches pluripotentes › Cellules souches pluripotentes induites › Generation of three human induced pluripotent...
questionsmedicales.fr › Cellules › Cellules musculaires › Myocytes cardiaques › Generation of three human induced pluripotent...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Facteurs de transcription › Generation of three human induced pluripotent...