The use of soft X-ray tomography to explore mitochondrial structure and function.

Mitochondria Tomography, X-Ray / methods
Cell mapping Mitochondria Spatial biology Structural biology Tomography

Journal

Molecular metabolism
ISSN: 2212-8778
Titre abrégé: Mol Metab
Pays: Germany
ID NLM: 101605730

Informations de publication

Date de publication:
03 2022
Historique:
received: 30 06 2021
revised: 22 11 2021
accepted: 15 12 2021
pubmed: 24 12 2021
medline: 5 4 2022
entrez: 23 12 2021
Statut: ppublish

Résumé

Mitochondria are cellular organelles responsible for energy production, and dysregulation of the mitochondrial network is associated with many disease states. To fully characterize the mitochondrial network's structure and function, a three-dimensional whole cell mapping technique is required. This review highlights the use of soft X-ray tomography (SXT) as a relatively high-throughput approach to quantify mitochondrial structure and function under multiple cellular conditions. The use of SXT opens the door for mapping cellular rearrangements during critical processes such as insulin secretion, stem cell differentiation, or disease progression. SXT provides unique information such as biochemical compositions or molecular densities of organelles and allows for unbiased, label-free imaging of intact whole cells. Mapping mitochondria in the context of the near-native cellular environment will reveal more information regarding mitochondrial network functions within the cell.

Sections du résumé

BACKGROUND
Mitochondria are cellular organelles responsible for energy production, and dysregulation of the mitochondrial network is associated with many disease states. To fully characterize the mitochondrial network's structure and function, a three-dimensional whole cell mapping technique is required.
SCOPE OF REVIEW
This review highlights the use of soft X-ray tomography (SXT) as a relatively high-throughput approach to quantify mitochondrial structure and function under multiple cellular conditions.
MAJOR CONCLUSIONS
The use of SXT opens the door for mapping cellular rearrangements during critical processes such as insulin secretion, stem cell differentiation, or disease progression. SXT provides unique information such as biochemical compositions or molecular densities of organelles and allows for unbiased, label-free imaging of intact whole cells. Mapping mitochondria in the context of the near-native cellular environment will reveal more information regarding mitochondrial network functions within the cell.

Identifiants

DOI: 10.1016/j.molmet.2021.101421 PMID: 34942399 PMC: PMC8829759
pubmed: 34942399
pii: S2212-8778(21)00279-9
doi: 10.1016/j.molmet.2021.101421
pmc: PMC8829759
pii:
doi:

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Review

Langues

eng

Sous-ensembles de citation

IM

Pagination

101421

Subventions

Organisme : NIGMS NIH HHS
ID : P30 GM138441
Pays : United States

Informations de copyright

Copyright © 2021 The Authors. Published by Elsevier GmbH.. All rights reserved.

Auteurs

Valentina Loconte (V)

Department of Anatomy, School of Medicine, UCSF, San Francisco, CA 94143, USA; Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.

Kate L White (KL)

Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA; Department of Chemistry, Bridge Institute, Michelson Center for Convergent Bioscience, University of Southern California, Los Angeles, CA 90089, USA. Electronic address: katewhit@usc.edu.

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Classifications MeSH

questionsmedicales.fr › Cellules › Structures cellulaires › Fractions subcellulaires › Mitochondries › The use of soft X-ray tomography to explore...
questionsmedicales.fr › Diagnostic › Techniques et procédures diagnostiques › Imagerie diagnostique › Tomographie › Tomographie à rayons X › The use of soft X-ray tomography to explore...