Rapid, efficient and activation-neutral gene editing of polyclonal primary human resting CD4
CD4-Positive T-Lymphocytes
/ cytology
CRISPR-Associated Protein 9
/ genetics
CRISPR-Cas Systems
/ genetics
Cell Movement
/ genetics
Cells, Cultured
DNA
Gene Editing
/ methods
Gene Knockout Techniques
HIV Infections
/ genetics
Humans
Membrane Glycoproteins
/ genetics
Myxovirus Resistance Proteins
/ genetics
RNA, Guide, Kinetoplastida
SAM Domain and HD Domain-Containing Protein 1
/ genetics
Transgenes
mRNA Cleavage and Polyadenylation Factors
/ genetics
Journal
Nature methods
ISSN: 1548-7105
Titre abrégé: Nat Methods
Pays: United States
ID NLM: 101215604
Informations de publication
Date de publication:
01 2022
01 2022
Historique:
received:
19
01
2021
accepted:
22
10
2021
pubmed:
25
12
2021
medline:
3
3
2022
entrez:
24
12
2021
Statut:
ppublish
Résumé
CD4
Identifiants
pubmed: 34949807
doi: 10.1038/s41592-021-01328-8
pii: 10.1038/s41592-021-01328-8
pmc: PMC8748193
doi:
Substances chimiques
MX2 protein, human
0
Membrane Glycoproteins
0
Myxovirus Resistance Proteins
0
P-selectin ligand protein
0
RNA, Guide
0
cleavage factor Im, human
0
mRNA Cleavage and Polyadenylation Factors
0
DNA
9007-49-2
CRISPR-Associated Protein 9
EC 3.1.-
SAM Domain and HD Domain-Containing Protein 1
EC 3.1.5.-
SAMHD1 protein, human
EC 3.1.5.-
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
81-89Subventions
Organisme : NIAID NIH HHS
ID : R01 AI145753
Pays : United States
Commentaires et corrections
Type : CommentIn
Informations de copyright
© 2021. The Author(s).
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