Live Fluorescence Imaging of Chromosome Segregation in Cultured Cells.
Cell division
Fluorescent protein
Light sheet fluorescence microscopy
Live-cell imaging
Microscope
Mitosis
Wide field fluorescence microscopy
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2022
2022
Historique:
entrez:
1
1
2022
pubmed:
2
1
2022
medline:
1
4
2022
Statut:
ppublish
Résumé
Live-cell fluorescence microscopy is an effective tool for characterizing aberrant mitotic phenotypes resulting from exposure to chemical inhibitors and after RNA interference-mediated or CRISPR knockout-mediated depletion of protein targets. Live imaging of cultured cells during mitotic progression presents challenges in maintaining optimal health of cells while achieving the temporal and spatial resolution to accomplish the goals of the study. Herein are strategies to monitor and analyze mammalian cell mitosis utilizing either a wide field or a light sheet, inverted, fluorescence microscope.
Identifiants
pubmed: 34972946
doi: 10.1007/978-1-0716-1904-9_5
pmc: PMC10092068
mid: NIHMS1885817
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
61-86Subventions
Organisme : NIGMS NIH HHS
ID : R35 GM126980
Pays : United States
Informations de copyright
© 2022. Springer Science+Business Media, LLC, part of Springer Nature.
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