Assessing the impact of substrate-level enzyme regulations limiting ethanol titer in Clostridium thermocellum using a core kinetic model.

Cellobiose / metabolism Clostridium thermocellum / genetics Ethanol / metabolism Fermentation Kinetics
Acetovibrio cellulyticus Clostridium thermocellum Ensemble docking Hungataiclostridium thermocellum Kinetic modeling Ruminiclostridium thermocellum

Journal

Metabolic engineering
ISSN: 1096-7184
Titre abrégé: Metab Eng
Pays: Belgium
ID NLM: 9815657

Informations de publication

Date de publication:
01 2022
Historique:
received: 27 09 2021
revised: 16 12 2021
accepted: 29 12 2021
pubmed: 5 1 2022
medline: 15 3 2022
entrez: 4 1 2022
Statut: ppublish

Résumé

Clostridium thermocellum is a promising candidate for consolidated bioprocessing because it can directly ferment cellulose to ethanol. Despite significant efforts, achieved yields and titers fall below industrially relevant targets. This implies that there still exist unknown enzymatic, regulatory, and/or possibly thermodynamic bottlenecks that can throttle back metabolic flow. By (i) elucidating internal metabolic fluxes in wild-type C. thermocellum grown on cellobiose via

Identifiants

DOI: 10.1016/j.ymben.2021.12.012 PMID: 34982997
pubmed: 34982997
pii: S1096-7176(21)00198-1
doi: 10.1016/j.ymben.2021.12.012
pii:
doi:

Substances chimiques

Cellobiose 16462-44-5
Ethanol 3K9958V90M

Types de publication

Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Langues

eng

Sous-ensembles de citation

IM

Pagination

286-301

Informations de copyright

Copyright © 2021. Published by Elsevier Inc.

Auteurs

Charles Foster (C)

Department of Chemical Engineering, The Pennsylvania State University, University Park, PA, USA; Center for Bioenergy Innovation, Oak Ridge, TN, USA.

Veda Sheersh Boorla (VS)

Department of Chemical Engineering, The Pennsylvania State University, University Park, PA, USA; Center for Bioenergy Innovation, Oak Ridge, TN, USA.

Satyakam Dash (S)

Department of Chemical Engineering, The Pennsylvania State University, University Park, PA, USA; Center for Bioenergy Innovation, Oak Ridge, TN, USA.

Saratram Gopalakrishnan (S)

Department of Chemical Engineering, The Pennsylvania State University, University Park, PA, USA; Center for Bioenergy Innovation, Oak Ridge, TN, USA.

Tyler B Jacobson (TB)

Department of Bacteriology, University of Wisconsin-Madison, Madison, WI, USA; Center for Bioenergy Innovation, Oak Ridge, TN, USA.

Daniel G Olson (DG)

Thayer School of Engineering at Dartmouth College, Hanover, NH, USA; Center for Bioenergy Innovation, Oak Ridge, TN, USA.

Daniel Amador-Noguez (D)

Department of Bacteriology, University of Wisconsin-Madison, Madison, WI, USA; Center for Bioenergy Innovation, Oak Ridge, TN, USA.

Lee R Lynd (LR)

Thayer School of Engineering at Dartmouth College, Hanover, NH, USA; Center for Bioenergy Innovation, Oak Ridge, TN, USA.

Costas D Maranas (CD)

Department of Chemical Engineering, The Pennsylvania State University, University Park, PA, USA; Center for Bioenergy Innovation, Oak Ridge, TN, USA. Electronic address: costas@psu.edu.

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Classifications MeSH

questionsmedicales.fr › Glucides › Sucres › Diholoside › Cellobiose › Assessing the impact of substrate-level enzyme...
questionsmedicales.fr › Bactéries › Bactéries à Gram positif › Bactéries à Gram positif formant des endospores › Clostridium › Clostridium thermocellum › Assessing the impact of substrate-level enzyme...
questionsmedicales.fr › Composés chimiques organiques › Alcools › Éthanol › Assessing the impact of substrate-level enzyme...
questionsmedicales.fr › Métabolisme › Métabolisme glucidique › Fermentation › Assessing the impact of substrate-level enzyme...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Cinétique › Assessing the impact of substrate-level enzyme...