Protein Preparation for Proteomic Analysis of the Unfolded Protein Response in Arabidopsis thaliana.
Arabidopsis
Endoplasmic reticulum stress
Protein extraction
Proteomics
Unfolded protein response
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2022
2022
Historique:
entrez:
5
1
2022
pubmed:
6
1
2022
medline:
8
3
2022
Statut:
ppublish
Résumé
Excessive accumulation of unfolded or misfolded proteins in the endoplasmic reticulum (ER) leads to a potentially cytotoxic condition known as the ER stress. Upon ER stress, cells initiate a homeostatic response called unfolded protein response (UPR) to assist proper folding the unfolded or misfolded proteins. Proteomics have been broadly used in plants with Liquid Chromatography coupled to tandem MS (LC-MS/MS) technologies. LC-MS/MS techniques have also been a great tool for studies of posttranslational modifications (PTMs). Here we describe our protocol of a fast method for large amount of seedling treatment and collection for UPR study in Arabidopsis thaliana and the preparation of total proteins for proteomic analysis.
Identifiants
pubmed: 34985707
doi: 10.1007/978-1-0716-1732-8_18
pmc: PMC8935445
mid: NIHMS1785421
doi:
Substances chimiques
Arabidopsis Proteins
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
279-289Subventions
Organisme : NIGMS NIH HHS
ID : R35 GM136637
Pays : United States
Informations de copyright
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
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