Individual copy number variation and extensive diversity between major MHC-DAB1 allelic lineages in the European bitterling.
Gene duplication
MHC class IIB
MHC supertypes
Positive selection
Positively selected sites
Rhodeus amarus
Journal
Immunogenetics
ISSN: 1432-1211
Titre abrégé: Immunogenetics
Pays: United States
ID NLM: 0420404
Informations de publication
Date de publication:
10 2022
10 2022
Historique:
received:
08
11
2021
accepted:
31
12
2021
pubmed:
12
1
2022
medline:
15
9
2022
entrez:
11
1
2022
Statut:
ppublish
Résumé
Polymorphism of the major histocompatibility complex (MHC), DAB1 gene was characterized for the first time in the European bitterling (Rhodeus amarus), a freshwater fish employed in studies of host-parasite coevolution and mate choice, taking advantage of newly designed primers coupled with high-throughput amplicon sequencing. Across 221 genotyped individuals, we detected 1-4 variants per fish, with 28% individuals possessing 3-4 variants. We identified 36 DAB1 variants, and they showed high sequence diversity mostly located within predicted antigen-binding sites, and both global and codon-specific excess of non-synonymous mutations. Despite deep divergence between two major allelic lineages, functional diversity was surprisingly low (3 supertypes). Overall, these findings suggest the role of positive and balancing selection in promotion and long-time maintenance of DAB1 polymorphism. Further investigations will clarify the role of pathogen-mediated selection to drive the evolution of DAB1 variation.
Identifiants
pubmed: 35015128
doi: 10.1007/s00251-021-01251-4
pii: 10.1007/s00251-021-01251-4
pmc: PMC9467946
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
497-505Informations de copyright
© 2022. The Author(s).
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