Differential LysoTracker Uptake Defines Two Populations of Distal Epithelial Cells in Idiopathic Pulmonary Fibrosis.
Alveolar Epithelial Cells
/ metabolism
Amines
/ metabolism
Animals
Biomarkers
/ metabolism
Bleomycin
CD24 Antigen
/ metabolism
Epithelium
/ pathology
Gene Expression Profiling
Humans
Idiopathic Pulmonary Fibrosis
/ genetics
Keratin-5
/ metabolism
Mice, Inbred C57BL
Pulmonary Surfactant-Associated Proteins
/ metabolism
Receptors, Nerve Growth Factor
/ metabolism
Tissue Donors
Transcription, Genetic
Up-Regulation
CD24
CK5
IPF
LysoTracker
NGFR
alveolar epithelial cells
flow cytometry
intermediate epithelial cells
lung transcriptomic profile
transitional states
Journal
Cells
ISSN: 2073-4409
Titre abrégé: Cells
Pays: Switzerland
ID NLM: 101600052
Informations de publication
Date de publication:
11 01 2022
11 01 2022
Historique:
received:
30
11
2021
revised:
26
12
2021
accepted:
04
01
2022
entrez:
21
1
2022
pubmed:
22
1
2022
medline:
1
3
2022
Statut:
epublish
Résumé
Idiopathic pulmonary fibrosis (IPF) is a progressive and fatal degenerative lung disease of unknown etiology. Although in its final stages it implicates, in a reactive manner, all lung cell types, the initial damage involves the alveolar epithelial compartment, in particular the alveolar epithelial type 2 cells (AEC2s). AEC2s serve dual progenitor and surfactant secreting functions, both of which are deeply impacted in IPF. Thus, we hypothesize that the size of the surfactant processing compartment, as measured by LysoTracker incorporation, allows the identification of different epithelial states in the IPF lung. Flow cytometry analysis of epithelial LysoTracker incorporation delineates two populations (Lyso
Identifiants
pubmed: 35053350
pii: cells11020235
doi: 10.3390/cells11020235
pmc: PMC8773634
pii:
doi:
Substances chimiques
Amines
0
Biomarkers
0
CD24 Antigen
0
Keratin-5
0
Pulmonary Surfactant-Associated Proteins
0
Receptors, Nerve Growth Factor
0
Red DND-99
0
Bleomycin
11056-06-7
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : German Center for Lung Research
ID : KFO309 P7, SFB CRC1213-project A04 and EL 931/4-1
Organisme : Bundes Ministerium für Bildung und Forschung
ID : RARE-ILD consortium (European Joint Program on Rare Diseases, EJP-RD)
Organisme : Lung Clinic Waldhof Elgershausen
ID : Lung Fibrosis Stipend
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