Belimumab Decreases Autophagy and Citrullination in Peripheral Blood Mononuclear Cells from Patients with Systemic Lupus Erythematosus.
Adult
Antibodies, Monoclonal, Humanized
/ pharmacology
Antigens, CD
/ metabolism
Autophagy
/ drug effects
B-Cell Activation Factor Receptor
/ metabolism
B-Cell Maturation Antigen
/ metabolism
Biomarkers
/ blood
Cell Membrane
/ drug effects
Citrullination
/ drug effects
Female
Humans
Interleukin-18
/ blood
Leukocytes, Mononuclear
/ drug effects
Lupus Erythematosus, Systemic
/ blood
Lysosomal Membrane Proteins
/ metabolism
Male
Microtubule-Associated Proteins
/ metabolism
Middle Aged
Transmembrane Activator and CAML Interactor Protein
/ metabolism
B lymphocyte stimulator (BLyS)
BLyS receptors
IL-18
autophagy
belimumab
citrullination
peptidylarginine deiminases (PADs)
systemic lupus erythematosus
Journal
Cells
ISSN: 2073-4409
Titre abrégé: Cells
Pays: Switzerland
ID NLM: 101600052
Informations de publication
Date de publication:
13 01 2022
13 01 2022
Historique:
received:
22
12
2021
accepted:
10
01
2022
entrez:
21
1
2022
pubmed:
22
1
2022
medline:
24
2
2022
Statut:
epublish
Résumé
Belimumab (BLM) is a B lymphocyte stimulator (BLyS) inhibitor approved for the treatment of systemic lupus erythematosus (SLE). Autophagy is a cell survival mechanism involved in the pathogenesis of SLE. Citrullination is a post-translational modification catalyzed by peptidylarginine deiminase (PAD) enzymes. Autophagy and citrullination may generate neoepitopes, evoking an autoimmune response. No previous studies have investigated the connection of these processes, and how BLM could affect them, in SLE. Ex vivo autophagy and protein citrullination were analyzed by western blot in lysates from 26 SLE patients' PBMCs at baseline and after 2, 4, and 12 weeks of BLM administration, and from 16 healthy donors' PBMCs. Autophagic PBMCs were identified by the immunofluorescent detection of the autophagy-associated proteins LC3B (LC3 puncta) and LAMP-1. Autophagosome accumulation was evaluated in CD14
Identifiants
pubmed: 35053379
pii: cells11020262
doi: 10.3390/cells11020262
pmc: PMC8773843
pii:
doi:
Substances chimiques
Antibodies, Monoclonal, Humanized
0
Antigens, CD
0
B-Cell Activation Factor Receptor
0
B-Cell Maturation Antigen
0
Biomarkers
0
Interleukin-18
0
LAMP1 protein, human
0
Lysosomal Membrane Proteins
0
MAP1LC3A protein, human
0
Microtubule-Associated Proteins
0
TNFRSF13C protein, human
0
Transmembrane Activator and CAML Interactor Protein
0
belimumab
73B0K5S26A
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Fondazione Umberto Di Mario
ID : N/A
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