Synergetic Fermentation of Glucose and Glycerol for High-Yield N-Acetylglucosamine Production in
Escherichia coli
N-acetylglucosamine
fructose-6-phosphate accumulation
metabolic engineering
synergetic carbon fermentation
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
11 Jan 2022
11 Jan 2022
Historique:
received:
18
10
2021
revised:
28
12
2021
accepted:
09
01
2022
entrez:
21
1
2022
pubmed:
22
1
2022
medline:
5
2
2022
Statut:
epublish
Résumé
N-acetylglucosamine (GlcNAc) is an amino sugar that has been widely used in the nutraceutical and pharmaceutical industries. Recently, microbial production of GlcNAc has been developed. One major challenge for efficient biosynthesis of GlcNAc is to achieve appropriate carbon flux distribution between growth and production. Here, a synergistic substrate co-utilization strategy was used to address this challenge. Specifically, glycerol was utilized to support cell growth and generate glutamine and acetyl-CoA, which are amino and acetyl donors, respectively, for GlcNAc biosynthesis, while glucose was retained for GlcNAc production. Thanks to deletion of the 6-phosphofructokinase (PfkA and PfkB) and glucose-6-phosphate dehydrogenase (ZWF) genes, the main glucose catabolism pathways of
Identifiants
pubmed: 35054959
pii: ijms23020773
doi: 10.3390/ijms23020773
pmc: PMC8775389
pii:
doi:
Substances chimiques
Culture Media
0
Glucose
IY9XDZ35W2
Glycerol
PDC6A3C0OX
Acetylglucosamine
V956696549
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : National Natural Science Foundation of China
ID : 32172767
Organisme : Agricultural Science and Technology Innovation Program
ID : cxgc-ias-16
Organisme : State Key Laboratory of Animal Nutrition Project
ID : 2004DA125184G2101
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