Modulating Expression of Endogenous Interleukin 1 Beta in the Acute Phase of the Pilocarpine Model of Epilepsy May Change Animal Survival.
Animal model
Mesial temporal lobe epilepsy
Neuroinflammation
RNA interference in vivo
Journal
Cellular and molecular neurobiology
ISSN: 1573-6830
Titre abrégé: Cell Mol Neurobiol
Pays: United States
ID NLM: 8200709
Informations de publication
Date de publication:
Jan 2023
Jan 2023
Historique:
received:
04
06
2021
accepted:
05
01
2022
pubmed:
22
1
2022
medline:
7
1
2023
entrez:
21
1
2022
Statut:
ppublish
Résumé
The pilocarpine-induced (PILO) model has helped elucidate the electrophysiological and molecular aspects related to mesial temporal lobe epilepsy. It has been suggested that the extensive cell death and edema observed in the brains of these animals could be induced by increased inflammatory responses, such as the rapid release of the inflammatory cytokine interleukin 1 beta (Il1b). In this study, we investigate the role of endogenous Il1b in the acute phase of the PILO model. Our aim is twofold. First, we want to determine whether it is feasible to silence Il1b in the central nervous system using a non-invasive procedure. Second, we aim to investigate the effect of silencing endogenous Il1b and its antagonist, Il1rn.We used RNA interference applied non-invasively to knockdown Il1b and its endogenous antagonist Il1rn. We found that knocking down Il1b prior to pilocarpine injection increased the mortality rate of treated animals. Furthermore, we observed that, when exposing the animals to more Il1b by silencing its endogenous antagonist Il1rn, there was a better response to status epilepticus with decreased animal mortality in the acute phase of the PILO model. Thus, we show the feasibility of using a novel, less invasive approach to study genes involved in the inflammatory response in the central nervous system. Furthermore, our results provide suggestive evidence that modulating endogenous Il1b improves animal survival in the acute phase of the PILO model and may have effects that extend into the chronic phase.
Identifiants
pubmed: 35061107
doi: 10.1007/s10571-022-01190-y
pii: 10.1007/s10571-022-01190-y
doi:
Substances chimiques
Pilocarpine
01MI4Q9DI3
Interleukin-1beta
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
367-380Subventions
Organisme : Fundação de Amparo à Pesquisa do Estado de São Paulo
ID : 2013/07559-3
Organisme : Fundação de Amparo à Pesquisa do Estado de São Paulo
ID : 2005/56663-1
Organisme : Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
ID : 001
Organisme : Conselho Nacional de Desenvolvimento Científico e Tecnológico
ID : 403299/2016-0
Organisme : Conselho Nacional de Desenvolvimento Científico e Tecnológico
ID : 309494/2014-1
Informations de copyright
© 2022. The Author(s).
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