Loop-mediated isothermal amplification (LAMP): a sensitive molecular tool for detection of Staphylococcus aureus in meat and dairy product.
Foodborne pathogens
Genes
LAMP
Staphylococcus aureus
arcC
femA
Journal
Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]
ISSN: 1678-4405
Titre abrégé: Braz J Microbiol
Pays: Brazil
ID NLM: 101095924
Informations de publication
Date de publication:
Mar 2022
Mar 2022
Historique:
received:
02
08
2021
accepted:
12
11
2021
pubmed:
30
1
2022
medline:
3
3
2022
entrez:
29
1
2022
Statut:
ppublish
Résumé
Staphylococcus aureus-mediated food poisoning is a primary concern worldwide. The presence of the organism in food is an indicative of poor sanitation during production, and it is essential to have efficient methods for detecting this pathogen. A novel molecular diagnostic technique called loop-mediated isothermal amplification (LAMP) serves as a rapid and sensitive detection method, which amplifies nucleic acids at isothermal conditions. In this study, a LAMP-based diagnostic assay was developed to detect Staphylococcus aureus (S. aureus) using two target genes femA and arcC. The optimum reaction temperature was found to be 65 °C and at 60 °C for femA and arcC genes, respectively. The developed assay specifically amplified DNA from S. aureus, not from other related bacterial species and compared to PCR, and a 100-fold higher sensitivity was observed. Furthermore, the LAMP assay could detect the pathogen from food samples mainly meat and dairy samples when analyzed in both intact and enriched conditions. Thirteen samples were found positive for S. aureus with LAMP showing a greater number of positive samples in comparison to PCR. This study established a highly sensitive and a rapid diagnostic procedure for the detection and surveillance of this major foodborne pathogen.
Identifiants
pubmed: 35091898
doi: 10.1007/s42770-021-00659-0
pii: 10.1007/s42770-021-00659-0
pmc: PMC8882521
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
341-347Subventions
Organisme : department of science and technology,india
ID : Department of Science
Organisme : department of science and technology,india
ID : Technology (DST)
Organisme : department of science and technology,india
ID : Government of India for providing the fund under women Scientist Scheme (WOS-A): SR/WOS-A/LS-1065/2014 (G).
Informations de copyright
© 2021. Sociedade Brasileira de Microbiologia.
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