Increasing-Aeration Strategy: a Practical Approach to Enhance the Schizophyllan Production and Improve the Operational Conditions of Schizophyllum commune Cultivation in the Stirred Tank and Bubble Column Bioreactors.
Bubble column bioreactor
Oxygen limitation
Schizophyllan
Schizophyllum commune
Stirred tank bioreactor
Journal
Applied biochemistry and biotechnology
ISSN: 1559-0291
Titre abrégé: Appl Biochem Biotechnol
Pays: United States
ID NLM: 8208561
Informations de publication
Date de publication:
May 2022
May 2022
Historique:
accepted:
30
11
2021
pubmed:
1
2
2022
medline:
7
5
2022
entrez:
31
1
2022
Statut:
ppublish
Résumé
In the present study, the effect of employing the increasing- aeration strategy (IAS) in the oxygen-limited situation and proportionate to increasing oxygen demand of the fungus Schizophyllum commune (S. commune) has been investigated in both stirred tank (STB) and bubble column (BCB) bioreactors. The purpose was to enhance schizophyllan (SPG) production by preventing oxygen starvation, improve mixing conditions of pseudoplastic culture, and intensify shear stress on fungus pellets to release SPG. At first, a constant-aeration rate of 0.08 vvm was implemented in both bioreactors to evaluate the new strategy compared to the previously studied methods. In the second set of experiments with IAS, along with the increasing oxygen demand of culture, the inlet airflow was increased gradually, while the dissolved oxygen (DO) was maintained higher than zero and below 1%. Using IAS in STB significantly raised productivity by about 100% in 96 h from 0.035 to 0.073 g/L.h. Also, employing this strategy in BCB led to a 30% increase in the maximum SPG production from 3.2 to 4.2 g/L. IAS can effectively help handle the operation of S. commune cultivation on a large scale by improving mixing conditions, mass transfer, and shear stress in both bioreactor types. This method had a significant impact on STB cultivation and its productivity so that it can be a practical approach to SPG's industrial production.
Identifiants
pubmed: 35099723
doi: 10.1007/s12010-021-03777-5
pii: 10.1007/s12010-021-03777-5
doi:
Substances chimiques
Sizofiran
7F763NNC9X
Oxygen
S88TT14065
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
2284-2300Informations de copyright
© 2021. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.
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