Sulfotransferase 4A1 activity facilitates sulfate-dependent cellular protection to oxidative stress.

Sulfates

Journal

Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288

Informations de publication

Date de publication:
31 01 2022
Historique:
received: 28 09 2021
accepted: 13 01 2022
entrez: 1 2 2022
pubmed: 2 2 2022
medline: 4 3 2022
Statut: epublish

Résumé

Sulfotransferase 4A1 (SULT4A1) is an orphan member of the cytosolic SULT superfamily that contains enzymes that catalyze the sulfonation of hydrophobic drugs and hormones. SULT4A1 has been assessed through all classical SULT approaches yet no SULT activity has been reported. To ascertain SULT4A1 function and activity, we utilized Saccharomyces cerevisiae as a model system, which exhibits no endogenous SULT activity nor possesses SULT-related genes. We observed that ectopic SULT4A1 expression in yeast displays similar subcellular localization as reported in mouse neurons and observed that SULT4A1 is associated with the outer mitochondria membrane. SULT4A1 expression stimulates colony formation and protects these cells from hydrogen peroxide and metabolism-associated oxidative stress. These SULT4A1-mediated phenotypes are dependent on extracellular sulfate that is converted in yeast to PAPS, the universal sulfonate donor for SULT activity. Thus, heterologous SULT4A1 expression in yeast is correctly distributed and functional, and SULT4A1 antioxidant activity is sulfate dependent supporting the concept that SULT4A1 has sulfate-associated activity.

Identifiants

DOI: 10.1038/s41598-022-05582-4 PMID: 35102205 PMC: PMC8803991
pubmed: 35102205
doi: 10.1038/s41598-022-05582-4
pii: 10.1038/s41598-022-05582-4
pmc: PMC8803991
doi:

Substances chimiques

Sulfates 0

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

1625

Subventions

Organisme : NINDS NIH HHS
ID : R21 NS116312
Pays : United States

Informations de copyright

© 2022. The Author(s).

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Auteurs

Evan J Brettrager (EJ)

Department of Pharmacology and Toxicology, University of Alabama at Birmingham, 155 Volker Hall, 1720 2nd Ave S., Birmingham, AL, 35294-0019, USA.

Arthur W Meehan (AW)

Department of Pharmacology and Toxicology, University of Alabama at Birmingham, 155 Volker Hall, 1720 2nd Ave S., Birmingham, AL, 35294-0019, USA.

Charles N Falany (CN)

Department of Pharmacology and Toxicology, University of Alabama at Birmingham, 155 Volker Hall, 1720 2nd Ave S., Birmingham, AL, 35294-0019, USA.

Robert C A M van Waardenburg (RCAM)

Department of Pharmacology and Toxicology, University of Alabama at Birmingham, 155 Volker Hall, 1720 2nd Ave S., Birmingham, AL, 35294-0019, USA. rvanwaar@uab.edu.

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Classifications MeSH

questionsmedicales.fr › Produits chimiques inorganiques › Sulfates › Sulfotransferase 4A1 activity facilitates...