Influence of codon optimization, promoter, and strain selection on the heterologous production of a β-fructofuranosidase from Aspergillus fijiensis ATCC 20611 in Pichia pastoris.
Journal
Folia microbiologica
ISSN: 1874-9356
Titre abrégé: Folia Microbiol (Praha)
Pays: United States
ID NLM: 0376757
Informations de publication
Date de publication:
Apr 2022
Apr 2022
Historique:
received:
31
05
2021
accepted:
01
01
2022
pubmed:
9
2
2022
medline:
23
3
2022
entrez:
8
2
2022
Statut:
ppublish
Résumé
Fructooligosaccharides (FOS) are compounds possessing various health properties and are added to functional foods as prebiotics. The commercial production of FOS is done through the enzymatic transfructolysation of sucrose by β-fructofuranosidases which is found in various organisms of which Aureobasidium pullulans and Aspergillus niger are the most well known. This study overexpressed two differently codon-optimized variations of the Aspergillus fijiensis β-fructofuranosidase-encoding gene (fopA) under the transcriptional control of either the alcohol oxidase (AOX1) or glyceraldehyde-3-phosphate dehydrogenase (GAP) promoters. When cultivated in shake flasks, the two codon-optimized variants displayed similar volumetric enzyme activities when expressed under control of the same promoter with the GAP strains producing 11.7 U/ml and 12.7 U/ml, respectively, and the AOX1 strains 95.8 U/ml and 98.6 U/ml, respectively. However, the highest production levels were achieved for both codon-optimized genes when expressed under control of the AOX1 promoter. The AOX1 promoter was superior to the GAP promoter in bioreactor cultivations for both codon-optimized genes with 13,702 U/ml and 2718 U/ml for the AOX1 promoter for ATUM and GeneArt
Identifiants
pubmed: 35133569
doi: 10.1007/s12223-022-00947-8
pii: 10.1007/s12223-022-00947-8
doi:
Substances chimiques
Codon
0
Recombinant Proteins
0
beta-Fructofuranosidase
EC 3.2.1.26
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
339-350Informations de copyright
© 2022. Institute of Microbiology, Academy of Sciences of the Czech Republic, v.v.i.
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