[Plant prime editing technique: a new genome editing tool for plants].
design of pegRNA
dual-pegRNA
optimization
plant prime editing
Journal
Sheng wu gong cheng xue bao = Chinese journal of biotechnology
ISSN: 1872-2075
Titre abrégé: Sheng Wu Gong Cheng Xue Bao
Pays: China
ID NLM: 9426463
Informations de publication
Date de publication:
25 Jan 2022
25 Jan 2022
Historique:
entrez:
10
2
2022
pubmed:
11
2
2022
medline:
12
2
2022
Statut:
ppublish
Résumé
The CRISPR/Cas9 based prime editing (PE) technique enables all 12 types of base substitutions and precise small DNA deletions or insertions without generating DNA double-strand breaks. Prime editing has been successfully applied in plants and plays important roles in plant precision breeding. Although plant prime editing (PPE) can substantially expand the scope and capabilities of precise genome editing in plants, its editing efficiency still needs to be further improved. Here, we review the development of PPE technique, and introduce structural composition, advantages and limitations of PPE. Strategies to improve the PPE editing efficiency, including the
Identifiants
pubmed: 35142116
doi: 10.13345/j.cjb.210291
doi:
Substances chimiques
DNA
9007-49-2
Types de publication
Journal Article
Review
Langues
chi
Sous-ensembles de citation
IM