Soft X-ray tomography to map and quantify organelle interactions at the mesoscale.

Imaging, Three-Dimensional / methods Insulin Mitochondria / ultrastructure Organelles Tomography, X-Ray / methods
3D cell mapping mesoscale analysis organelle interaction pancreatic β cell soft X-ray tomography

Journal

Structure (London, England : 1993)
ISSN: 1878-4186
Titre abrégé: Structure
Pays: United States
ID NLM: 101087697

Informations de publication

Date de publication:
07 04 2022
Historique:
received: 19 08 2021
revised: 04 12 2021
accepted: 17 01 2022
pubmed: 13 2 2022
medline: 13 4 2022
entrez: 12 2 2022
Statut: ppublish

Résumé

Inter-organelle interactions are a vital part of normal cellular function; however, these have proven difficult to quantify due to the range of scales encountered in cell biology and the throughput limitations of traditional imaging approaches. Here, we demonstrate that soft X-ray tomography (SXT) can be used to rapidly map ultrastructural reorganization and inter-organelle interactions in intact cells. SXT takes advantage of the naturally occurring, differential X-ray absorption of the carbon-rich compounds in each organelle. Specifically, we use SXT to map the spatiotemporal evolution of insulin vesicles and their co-localization and interaction with mitochondria in pancreatic β cells during insulin secretion and in response to different stimuli. We quantify changes in the morphology, biochemical composition, and relative position of mitochondria and insulin vesicles. These findings highlight the importance of a comprehensive and unbiased mapping at the mesoscale to characterize cell reorganization that would be difficult to detect with other existing methodologies.

Identifiants

DOI: 10.1016/j.str.2022.01.006 PMID: 35148829 PMC: PMC9013509
pubmed: 35148829
pii: S0969-2126(22)00006-5
doi: 10.1016/j.str.2022.01.006
pmc: PMC9013509
mid: NIHMS1778529
pii:
doi:

Substances chimiques

Insulin 0

Types de publication

Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

510-521.e3

Subventions

Organisme : NIGMS NIH HHS
ID : P30 GM138441
Pays : United States
Organisme : NIGMS NIH HHS
ID : P41 GM103445
Pays : United States
Organisme : NIGMS NIH HHS
ID : P41 GM109824
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM083960
Pays : United States

Informations de copyright

Copyright © 2022 The Authors. Published by Elsevier Ltd.. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of interests The authors declare no competing interests.

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Auteurs

Valentina Loconte (V)

iHuman Institute, School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China.

Jitin Singla (J)

Department of Chemistry, Bridge Institute, USC Michelson Center for Convergent Bioscience, University of Southern California, Los Angeles, CA 90089, USA.

Angdi Li (A)

iHuman Institute, School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China.

Jian-Hua Chen (JH)

Department of Anatomy, University of California San Francisco, San Francisco, CA 94143, USA; Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.

Axel Ekman (A)

Department of Anatomy, University of California San Francisco, San Francisco, CA 94143, USA; Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.

Gerry McDermott (G)

Department of Anatomy, University of California San Francisco, San Francisco, CA 94143, USA; Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.

Andrej Sali (A)

Department of Bioengineering and Therapeutic Science, Department of Pharmaceutical Chemistry, California Institute of Quantitative Bioscience, University of California San Francisco, San Francisco, CA 94158, USA.

Mark Le Gros (M)

Department of Anatomy, University of California San Francisco, San Francisco, CA 94143, USA; Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.

Kate L White (KL)

Department of Chemistry, Bridge Institute, USC Michelson Center for Convergent Bioscience, University of Southern California, Los Angeles, CA 90089, USA. Electronic address: katewhit@usc.edu.

Carolyn A Larabell (CA)

Department of Anatomy, University of California San Francisco, San Francisco, CA 94143, USA; Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA. Electronic address: carolyn.larabell@ucsf.edu.

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Classifications MeSH

questionsmedicales.fr › Sciences de l'information › Méthodologies informatiques › Traitement d'image par ordinateur › Imagerie tridimensionnelle › Soft X-ray tomography to map and quantify...
questionsmedicales.fr › Acides aminés, peptides et protéines › Peptides › Hormones peptidiques › Hormones pancréatiques › Insulines › Insuline › Soft X-ray tomography to map and quantify...
questionsmedicales.fr › Cellules › Structures cellulaires › Fractions subcellulaires › Mitochondries › Soft X-ray tomography to map and quantify...
questionsmedicales.fr › Cellules › Structures cellulaires › Espace intracellulaire › Cytoplasme › Structures cytoplasmiques › Organites › Soft X-ray tomography to map and quantify...
questionsmedicales.fr › Diagnostic › Techniques et procédures diagnostiques › Imagerie diagnostique › Tomographie › Tomographie à rayons X › Soft X-ray tomography to map and quantify...