Inhibition of Full Smooth Muscle Contraction in Isolated Human Detrusor Tissues by Mirabegron Is Limited to Off-Target Inhibition of Neurogenic Contractions.
15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid
/ metabolism
Acetanilides
Carbachol
/ pharmacology
Endothelin-1
/ pharmacology
Female
Humans
Male
Methacholine Chloride
/ metabolism
Muscle Contraction
Muscle, Smooth
Receptors, Adrenergic
/ metabolism
Thiazoles
Urinary Bladder, Overactive
/ drug therapy
Journal
The Journal of pharmacology and experimental therapeutics
ISSN: 1521-0103
Titre abrégé: J Pharmacol Exp Ther
Pays: United States
ID NLM: 0376362
Informations de publication
Date de publication:
05 2022
05 2022
Historique:
received:
19
11
2021
accepted:
08
02
2022
pubmed:
15
2
2022
medline:
4
5
2022
entrez:
14
2
2022
Statut:
ppublish
Résumé
Mirabegron is used for treatment of storage symptoms in overactive bladder (OAB) caused by spontaneous bladder smooth muscle contractions. However, owing to limitations in available studies using human tissues, central questions are still unresolved, including mechanisms underlying improvements by mirabegron and its anticontractile effects in the detrusor. Here, we assessed concentration-dependent mirabegron effects on contractions of human detrusor tissues in frequency-response curves and concentration-response curves for different cholinergic and noncholinergic agonists. Detrusor tissues were sampled from patients undergoing radical cystectomy. Contractions were induced by electric field stimulation (EFS) and by cumulative concentrations of cholinergic agonists, endothelin-1, and the thromboxane A
Identifiants
pubmed: 35153197
pii: jpet.121.001029
doi: 10.1124/jpet.121.001029
doi:
Substances chimiques
Acetanilides
0
Endothelin-1
0
Receptors, Adrenergic
0
Thiazoles
0
Methacholine Chloride
0W5ETF9M2K
15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid
76898-47-0
Carbachol
8Y164V895Y
mirabegron
MVR3JL3B2V
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
176-187Informations de copyright
Copyright © 2022 by The American Society for Pharmacology and Experimental Therapeutics.