Biological cellulose saccharification using a coculture of Clostridium thermocellum and Thermobrachium celere strain A9.
Biological saccharification
Caloramator celer
Clostridium thermocellum
Glucose tolerance
Thermobrachium celere
Thermostability
β-Glucosidase
Journal
Applied microbiology and biotechnology
ISSN: 1432-0614
Titre abrégé: Appl Microbiol Biotechnol
Pays: Germany
ID NLM: 8406612
Informations de publication
Date de publication:
Mar 2022
Mar 2022
Historique:
received:
13
10
2021
accepted:
30
01
2022
revised:
30
12
2021
pubmed:
15
2
2022
medline:
22
3
2022
entrez:
14
2
2022
Statut:
ppublish
Résumé
An anaerobic thermophilic bacterial strain, A9 (NITE P-03545), that secretes β-glucosidase was newly isolated from wastewater sediments by screening using esculin. The 16S rRNA gene sequence of strain A9 had 100% identity with that of Thermobrachium celere type strain JW/YL-NZ35. The complete genome sequence of strain A9 showed 98.4% average nucleotide identity with strain JW/YL-NZ35. However, strain A9 had different physiological properties from strain JW/YL-NZ35, which cannot secrete β-glucosidases or grow on cellobiose as the sole carbon source. The key β-glucosidase gene (TcBG1) of strain A9, which belongs to glycoside hydrolase family 1, was characterized. Recombinant β-glucosidase (rTcBG1) hydrolyzed cellooligosaccharides to glucose effectively. Furthermore, rTcBG1 showed high thermostability (at 60°C for 2 days) and high glucose tolerance (IC
Identifiants
pubmed: 35157106
doi: 10.1007/s00253-022-11818-0
pii: 10.1007/s00253-022-11818-0
pmc: PMC8930880
doi:
Substances chimiques
RNA, Ribosomal, 16S
0
Cellulose
9004-34-6
beta-Glucosidase
EC 3.2.1.21
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
2133-2145Subventions
Organisme : Japan Science and Technology Corporation
ID : JPMJER1502
Organisme : Japan Science and Technology Corporation
ID : JPMJSA1801
Organisme : King Mongkut's University of Technology Thonburi
ID : 7601.24/4054
Organisme : National Major Science and Technology Projects of China
ID : 21-1-2-23-hz
Informations de copyright
© 2022. The Author(s).
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