Highly Sensitive "Off/On" EPR Probes to Monitor Enzymatic Activity.
EPR spectroscopy
carboxylesterase
enzymatic activity
micelles
nitroxide radicals
Journal
Chemistry (Weinheim an der Bergstrasse, Germany)
ISSN: 1521-3765
Titre abrégé: Chemistry
Pays: Germany
ID NLM: 9513783
Informations de publication
Date de publication:
22 Mar 2022
22 Mar 2022
Historique:
received:
23
12
2021
pubmed:
18
2
2022
medline:
24
3
2022
entrez:
17
2
2022
Statut:
ppublish
Résumé
The assessment of unregulated level of enzyme activity is a crucial parameter for early diagnoses in a wide range of pathologies. In this study, we propose the use of electron paramagnetic resonance (EPR) as an easy method to probe carboxylesterase (CE) enzymatic activity in vitro. For this application, were synthesized two amphiphilic, nitroxide containing esters, namely Tempo-C12 (T-C12) and Tempo-2-C12 (T-2-C12). They exhibit low solubility in water and form stable micelles in which the radicals are EPR almost silent, but the hydrolysis of the ester bond yields narrows and intense EPR signals. The intensity of the EPR signals is proportional to the enzymatic activity. CEs1, CEs2 and esterase from porcine liver (PLE) were investigated. The obtained results show that T-C12 and T-2-C12-containing systems display a much higher selectivity toward the CEs2, with a Limit of Detection of the same order of those ones obtained with optical methods.
Identifiants
pubmed: 35175676
doi: 10.1002/chem.202104563
pmc: PMC9314618
doi:
Substances chimiques
Esters
0
Carboxylesterase
EC 3.1.1.1
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e202104563Subventions
Organisme : Horizon 2020
ID : 86309 Primogaia
Informations de copyright
© 2022 The Authors. Chemistry - A European Journal published by Wiley-VCH GmbH.
Références
Protein Pept Lett. 2009;16(10):1207-14
pubmed: 19508181
PLoS One. 2009;4(4):e5244
pubmed: 19396361
J Mater Chem B. 2021 Mar 17;9(10):2457-2461
pubmed: 33630990
J Med Chem. 2009 Dec 10;52(23):7678-88
pubmed: 19634903
J Mater Chem B. 2018 Mar 21;6(11):1595-1599
pubmed: 32254275
J Am Chem Soc. 2008 Sep 10;130(36):11878-9
pubmed: 18700763
Food Chem. 2016 Feb 1;192:395-401
pubmed: 26304365
ACS Omega. 2021 May 28;6(23):15012-15016
pubmed: 34151082
Exp Cell Res. 2020 Apr 1;389(1):111856
pubmed: 31981591
J Med Chem. 2009 Jun 25;52(12):3742-52
pubmed: 19534556
Chem Commun (Camb). 2021 Sep 21;57(75):9602-9605
pubmed: 34546243
Chemistry. 2022 Mar 22;28(17):e202104563
pubmed: 35175676
Proteomics. 2009 Aug;9(16):3989-99
pubmed: 19658107
Angew Chem Int Ed Engl. 2015 Nov 2;54(45):13379-84
pubmed: 26376730
Expert Opin Drug Metab Toxicol. 2010 May;6(5):543-54
pubmed: 20192903
Chemistry. 2018 May 28;24(30):7615-7619
pubmed: 29722459
Contrast Media Mol Imaging. 2012 Jan-Feb;7(1):45-50
pubmed: 22344879
Anal Chem. 2020 Jul 7;92(13):9205-9213
pubmed: 32512997
J Pediatr Surg. 2013 Mar;48(3):502-9
pubmed: 23480903
Anal Chem. 2019 May 7;91(9):5504-5507
pubmed: 31013060
Chemistry. 2015 Jun 26;21(27):9645-9
pubmed: 26033618
Cancer Biol Ther. 2018 Mar 4;19(3):153-159
pubmed: 29261002
Life Sci. 2007 Aug 23;81(11):924-32
pubmed: 17764701
Annu Rev Anal Chem (Palo Alto Calif). 2018 Jun 12;11(1):509-533
pubmed: 29505726
Clin Cancer Res. 2013 Mar 1;19(5):1159-68
pubmed: 23325581
NMR Biomed. 2018 May;31(5):e3896
pubmed: 29493032
JCO Precis Oncol. 2020 Nov;4:426-436
pubmed: 35050739
Free Radic Biol Med. 2018 Oct;126:101-112
pubmed: 30092349
J Phys Chem B. 2020 Oct 1;124(39):8601-8609
pubmed: 32872777
Acta Pharm Sin B. 2018 Sep;8(5):699-712
pubmed: 30245959
Pharm Res. 2004 Nov;21(11):2017-24
pubmed: 15587923
Angew Chem Int Ed Engl. 2018 Sep 3;57(36):11701-11705
pubmed: 30003653
Clin Pharmacol Ther. 2004 Dec;76(6):528-35
pubmed: 15592324
Lung Cancer. 2003 Aug;41(2):187-98
pubmed: 12871782
Chem Sci. 2019 Jul 29;10(36):8428-8437
pubmed: 31803422
Magn Reson Chem. 2016 Nov;54(11):870-873
pubmed: 27412810