Early developmental, meiosis-specific proteins - Spo11, Msh4-1, and Msh5 - Affect subsequent genome reorganization in Paramecium tetraurelia.

Germ Cells Macronucleus / genetics Meiosis / genetics Paramecium tetraurelia / genetics RNA, Untranslated / metabolism
Genome rearrangement Meiosis Msh4 Msh5 Paramecium Spo11

Journal

Biochimica et biophysica acta. Molecular cell research
ISSN: 1879-2596
Titre abrégé: Biochim Biophys Acta Mol Cell Res
Pays: Netherlands
ID NLM: 101731731

Informations de publication

Date de publication:
06 2022
Historique:
received: 20 09 2021
revised: 07 02 2022
accepted: 10 02 2022
pubmed: 20 2 2022
medline: 19 4 2022
entrez: 19 2 2022
Statut: ppublish

Résumé

Developmental DNA elimination in Paramecium tetraurelia occurs through a trans-nuclear comparison of the genomes of two distinct types of nuclei: the germline micronucleus (MIC) and the somatic macronucleus (MAC). During sexual reproduction, which starts with meiosis of the germline nuclei, MIC-limited sequences including Internal Eliminated Sequences (IESs) and transposons are eliminated from the developing MAC in a process guided by noncoding RNAs (scnRNAs and iesRNAs). However, our current understanding of this mechanism is still very limited. Therefore, studying both genetic and epigenetic aspects of these processes is a crucial step to understand this phenomenon in more detail. Here, we describe the involvement of homologs of classical meiotic proteins, Spo11, Msh4-1, and Msh5 in this phenomenon. Based on our analyses, we propose that proper functioning of Spo11, Msh4-1, and Msh5 during Paramecium sexual reproduction are necessary for genome reorganization and viable progeny. Also, we show that double-strand breaks (DSBs) in DNA induced during meiosis by Spo11 are crucial for proper IESs excision. In summary, our investigations show that early sexual reproduction processes may significantly influence later somatic genome integrity.

Identifiants

DOI: 10.1016/j.bbamcr.2022.119239 PMID: 35181406
pubmed: 35181406
pii: S0167-4889(22)00030-1
doi: 10.1016/j.bbamcr.2022.119239
pii:
doi:

Substances chimiques

RNA, Untranslated 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

119239

Informations de copyright

Copyright © 2022 The Authors. Published by Elsevier B.V. All rights reserved.

Auteurs

Iwona Rzeszutek (I)

Institute of Cell Biology, University of Bern, Baltzerstrasse 4, 3012 Bern, Switzerland; Graduate School for Cellular and Biomedical Sciences, University of Bern, Freiestrasse 1, 3012 Bern, Switzerland; Institute of Biology and Biotechnology, Department of Biotechnology, University of Rzeszow, Pigonia 1, 35-310 Rzeszow, Poland.

Estienne C Swart (EC)

Institute of Cell Biology, University of Bern, Baltzerstrasse 4, 3012 Bern, Switzerland; Max Planck Institute for Developmental Biology, Max-Planck-Ring 5, 72076 Tuebingen, Germany.

Sylwia Pabian-Jewuła (S)

Institute of Cell Biology, University of Bern, Baltzerstrasse 4, 3012 Bern, Switzerland; Centre of Postgraduate Medical Education, Department of Clinical Cytology, Marymoncka 99/103, 01-813 Warsaw, Poland.

Antonietta Russo (A)

Institute of Cell Biology, University of Bern, Baltzerstrasse 4, 3012 Bern, Switzerland; Medical Biochemistry and Molecular Biology Department, UKS, Saarland Medical Center, Kirrberger Str. 100, 66421 Homburg, Germany.

Mariusz Nowacki (M)

Institute of Cell Biology, University of Bern, Baltzerstrasse 4, 3012 Bern, Switzerland. Electronic address: mariusz.nowacki@izb.unibe.ch.

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Classifications MeSH

questionsmedicales.fr › Cellules › Cellules germinales › Early developmental, meiosis-specific proteins...
questionsmedicales.fr › Cellules › Structures cellulaires › Espace intracellulaire › Cytoplasme › Structures cytoplasmiques › Organites › Noyau de la cellule › Macronucléus › Early developmental, meiosis-specific proteins...
questionsmedicales.fr › Phénomènes génétiques › Division du noyau cellulaire › Méiose › Early developmental, meiosis-specific proteins...
questionsmedicales.fr › Eucaryotes › Alveolata › Ciliophora › Oligohymenophorea › Hymenostomatida › Peniculina › Paramecium › Paramecium tetraurella › Early developmental, meiosis-specific proteins...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ARN › ARN non traduit › Early developmental, meiosis-specific proteins...