FFF-based high-throughput sequence shortlisting to support the development of aptamer-based analytical strategies.
Aptamer selectivity
Aptamers
Field-flow fractionation
Lysozyme
Screening method
Journal
Analytical and bioanalytical chemistry
ISSN: 1618-2650
Titre abrégé: Anal Bioanal Chem
Pays: Germany
ID NLM: 101134327
Informations de publication
Date de publication:
Jul 2022
Jul 2022
Historique:
received:
11
12
2021
accepted:
08
02
2022
revised:
05
02
2022
pubmed:
20
2
2022
medline:
2
7
2022
entrez:
19
2
2022
Statut:
ppublish
Résumé
Aptamers are biomimetic receptors that are increasingly exploited for the development of optical and electrochemical aptasensors. They are selected in vitro by the SELEX (Systematic Evolution of Ligands by Exponential Enrichment) procedure, but although they are promising recognition elements, for their reliable applicability for analytical purposes, one cannot ignore sample components that cause matrix effects. This particularly applies when different SELEX-selected aptamers and related truncated sequences are available for a certain target, and the choice of the aptamer should be driven by the specific downstream application. In this context, the present work aimed at investigating the potentialities of asymmetrical flow field-flow fractionation (AF4) with UV detection for the development of a screening method of a large number of anti-lysozyme aptamers towards lysozyme, including randomized sequences and an interfering agent (serum albumin). The possibility to work in native conditions and selectively monitor the evolution of untagged aptamer signal as a result of aptamer-protein binding makes the devised method effective as a strategy for shortlisting the most promising aptamers both in terms of affinity and in terms of selectivity, to support subsequent development of aptamer-based analytical devices.
Identifiants
pubmed: 35182166
doi: 10.1007/s00216-022-03971-2
pii: 10.1007/s00216-022-03971-2
pmc: PMC9242963
doi:
Substances chimiques
Aptamers, Nucleotide
0
Ligands
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
5519-5527Subventions
Organisme : Ministero dell'Istruzione, dell'Università e della Ricerca
ID : PRIN 2017 (Progetti di Ricerca di Rilevante Intere
Informations de copyright
© 2022. The Author(s).
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