The S-layer homology domains of Paenibacillus alvei surface protein SpaA bind to cell wall polysaccharide through the terminal monosaccharide residue.

Cell Wall / chemistry Ligands Membrane Glycoproteins / genetics Membrane Proteins / metabolism Monosaccharides / metabolism Paenibacillus / chemistry Polysaccharides / metabolism Protein Domains
S-layer SLH domain X-ray crystallography cell wall anchoring isothermal titration calorimetry secondary cell wall polymer

Journal

The Journal of biological chemistry
ISSN: 1083-351X
Titre abrégé: J Biol Chem
Pays: United States
ID NLM: 2985121R

Informations de publication

Date de publication:
04 2022
Historique:
received: 16 12 2021
revised: 16 02 2022
accepted: 17 02 2022
pubmed: 22 2 2022
medline: 27 4 2022
entrez: 21 2 2022
Statut: ppublish

Résumé

Self-assembling (glyco)protein surface layers (S-layers) are ubiquitous prokaryotic cell-surface structures involved in structural maintenance, nutrient diffusion, host adhesion, virulence, and other processes, which makes them appealing targets for therapeutics and biotechnological applications as biosensors or drug delivery systems. However, unlocking this potential requires expanding our understanding of S-layer properties, especially the details of surface-attachment. S-layers of Gram-positive bacteria often are attached through the interaction of S-layer homology (SLH) domain trimers with peptidoglycan-linked secondary cell wall polymers (SCWPs). Cocrystal structures of the SLH domain trimer from the Paenibacillus alvei S-layer protein SpaA (SpaA

Identifiants

DOI: 10.1016/j.jbc.2022.101745 PMID: 35189140 PMC: PMC8942822
pubmed: 35189140
pii: S0021-9258(22)00185-5
doi: 10.1016/j.jbc.2022.101745
pmc: PMC8942822
pii:
doi:

Substances chimiques

Ligands 0
Membrane Glycoproteins 0
Membrane Proteins 0
Monosaccharides 0
Polysaccharides 0
S-layer proteins 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

101745

Subventions

Organisme : Austrian Science Fund FWF
ID : W 1224
Pays : Austria

Informations de copyright

Copyright © 2022 The Authors. Published by Elsevier Inc. All rights reserved.

Déclaration de conflit d'intérêts

Conflict of interest The authors declare that they have no conflicts of interest with the contents of this article.

Auteurs

Max S G Legg (MSG)

Department of Biochemistry & Microbiology, University of Victoria, Victoria, British Columbia, Canada.

Fiona F Hager-Mair (FF)

Department of NanoBiotechnology, NanoGlycobiology Unit, Universität für Bodenkultur Wien, Vienna, Austria.

Simon Krauter (S)

Department of Chemistry, Institute of Organic Chemistry, Universität für Bodenkultur Wien, Vienna, Austria.

Susannah M L Gagnon (SML)

Department of Biochemistry & Microbiology, University of Victoria, Victoria, British Columbia, Canada.

Arturo Lòpez-Guzmán (A)

Department of NanoBiotechnology, NanoGlycobiology Unit, Universität für Bodenkultur Wien, Vienna, Austria.

Charlie Lim (C)

Department of Chemistry, Institute of Organic Chemistry, Universität für Bodenkultur Wien, Vienna, Austria.

Markus Blaukopf (M)

Department of Chemistry, Institute of Organic Chemistry, Universität für Bodenkultur Wien, Vienna, Austria.

Paul Kosma (P)

Department of Chemistry, Institute of Organic Chemistry, Universität für Bodenkultur Wien, Vienna, Austria.

Christina Schäffer (C)

Department of NanoBiotechnology, NanoGlycobiology Unit, Universität für Bodenkultur Wien, Vienna, Austria.

Stephen V Evans (SV)

Department of Biochemistry & Microbiology, University of Victoria, Victoria, British Columbia, Canada. Electronic address: svevans@uvic.ca.

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Classifications MeSH

questionsmedicales.fr › Cellules › Structures cellulaires › Paroi cellulaire › The S-layer homology domains of Paenibacillus...
questionsmedicales.fr › Actions chimiques et utilisations › Utilisations spécialisées de produits chimiques › Produits chimiques de laboratoire › Ligands › The S-layer homology domains of Paenibacillus...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines membranaires › Glycoprotéines membranaires › The S-layer homology domains of Paenibacillus...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines membranaires › The S-layer homology domains of Paenibacillus...
questionsmedicales.fr › Glucides › Sucres › Oses › The S-layer homology domains of Paenibacillus...
questionsmedicales.fr › Bactéries › Bactéries à Gram positif › Bâtonnets à Gram positif › Bâtonnets sporulés à Gram positif › Paenibacillus › The S-layer homology domains of Paenibacillus...
questionsmedicales.fr › Glucides › Polyosides › The S-layer homology domains of Paenibacillus...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Conformation moléculaire › Conformation des protéines › Structure tertiaire des protéines › Domaines protéiques › The S-layer homology domains of Paenibacillus...