Calcium imaging and BAPTA loading of amygdala astrocytes in mouse brain slices.
Cell Biology
Microscopy
Molecular/Chemical Probes
Neuroscience
Journal
STAR protocols
ISSN: 2666-1667
Titre abrégé: STAR Protoc
Pays: United States
ID NLM: 101769501
Informations de publication
Date de publication:
18 03 2022
18 03 2022
Historique:
entrez:
24
2
2022
pubmed:
25
2
2022
medline:
9
4
2022
Statut:
epublish
Résumé
Astrocytes are glial cells that exhibit calcium signaling-mediated activity. Here, we present a protocol to monitor and manipulate astrocyte calcium activity from mouse amygdala slices. In the first part of this protocol, we describe the procedure of astrocyte calcium imaging. In the second part, we detail how to disrupt astrocyte calcium activity by patch-clamp-mediated loading of BAPTA. These two approaches are presented separately but they can also be used simultaneously to monitor the effects of disruption on an astrocyte network. For complete details on the use and execution of this protocol, please refer to Wahis et al. (2021).
Identifiants
pubmed: 35199029
doi: 10.1016/j.xpro.2022.101159
pii: S2666-1667(22)00039-9
pmc: PMC8844720
doi:
Substances chimiques
Calcium, Dietary
0
Egtazic Acid
526U7A2651
1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid
K22DDW77C0
Calcium
SY7Q814VUP
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
101159Informations de copyright
© 2022 The Author(s).
Déclaration de conflit d'intérêts
The authors declare no competing interests.
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