Innovative method to grow the probiotic Lactobacillus reuteri in the omega3-rich microalga Isochrysis galbana.
Batch Cell Culture Techniques
/ methods
Culture Media
/ chemistry
Docosahexaenoic Acids
/ chemistry
Eicosapentaenoic Acid
/ chemistry
Fatty Acids, Omega-3
Fatty Acids, Unsaturated
/ chemistry
Fermentation
Haptophyta
/ metabolism
Limosilactobacillus reuteri
/ growth & development
Microalgae
/ chemistry
Probiotics
/ metabolism
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
24 02 2022
24 02 2022
Historique:
received:
26
05
2021
accepted:
11
02
2022
entrez:
25
2
2022
pubmed:
26
2
2022
medline:
8
3
2022
Statut:
epublish
Résumé
Microalgae are natural sources of valuable bioactive compounds, such as polyunsaturated fatty acids (PUFAs), that show antioxidant, anti-inflammatory, anticancer and antimicrobial activities. The marine microalga Isochrysis galbana (I. galbana) is extremely rich in ω3 PUFAs, mainly eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). Probiotics are currently suggested as adjuvant therapy in the management of diseases associated with gut dysbiosis. The Lactobacillus reuteri (L. reuteri), one of the most widely used probiotics, has been shown to produce multiple beneficial effects on host health. The present study aimed to present an innovative method for growing the probiotic L. reuteri in the raw seaweed extracts from I. galbana as an alternative to the conventional medium, under conditions of oxygen deprivation (anaerobiosis). As a result, the microalga I. galbana was shown for the first time to be an excellent culture medium for growing L. reuteri. Furthermore, the gas-chromatography mass-spectrometry analysis showed that the microalga-derived ω3 PUFAs were still available after the fermentation by L. reuteri. Accordingly, the fermented compound (FC), obtained from the growth of L. reuteri in I. galbana in anaerobiosis, was able to significantly reduce the adhesiveness and invasiveness of the harmful adherent-invasive Escherichia coli to intestinal epithelial cells, due to a cooperative effect between L. reuteri and microalgae-released ω3 PUFAs. These findings open new perspectives in the use of unicellular microalgae as growth medium for probiotics and in the production of biofunctional compounds.
Identifiants
pubmed: 35210548
doi: 10.1038/s41598-022-07227-y
pii: 10.1038/s41598-022-07227-y
pmc: PMC8873227
doi:
Substances chimiques
Culture Media
0
Fatty Acids, Omega-3
0
Fatty Acids, Unsaturated
0
Docosahexaenoic Acids
25167-62-8
Eicosapentaenoic Acid
AAN7QOV9EA
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
3127Informations de copyright
© 2022. The Author(s).
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