Physically interacting beta-delta pairs in the regenerating pancreas revealed by single-cell sequencing.
Endocrine pancreas
Islet of Langerhans
Pancreas regeneration
Pancreatectomy
Single-cell RNA-sequencing
Single-cell transcriptome sequencing
beta-delta cell pair
scRNA-seq
Journal
Molecular metabolism
ISSN: 2212-8778
Titre abrégé: Mol Metab
Pays: Germany
ID NLM: 101605730
Informations de publication
Date de publication:
06 2022
06 2022
Historique:
received:
24
10
2021
revised:
05
02
2022
accepted:
25
02
2022
pubmed:
4
3
2022
medline:
11
5
2022
entrez:
3
3
2022
Statut:
ppublish
Résumé
Until recently, communication between neighboring cells in islets of Langerhans was overlooked by genomic technologies, which require rigorous tissue dissociation into single cells. We utilize sorting of physically interacting cells (PICs) with single-cell RNA-sequencing to systematically map cellular interactions in the endocrine pancreas after pancreatectomy. The pancreas cellular landscape features pancreatectomy associated heterogeneity of beta-cells, including an interaction-specific program between paired beta and delta-cells. Our analysis suggests that the particular cluster of beta-cells that pairs with delta-cells benefits from stress protection, implying that the interaction between beta- and delta-cells might safeguard against pancreatectomy associated challenges. The work encourages testing the potential relevance of physically-interacting beta-delta-cells also in diabetes mellitus.
Identifiants
pubmed: 35240340
pii: S2212-8778(22)00036-9
doi: 10.1016/j.molmet.2022.101467
pmc: PMC8983436
pii:
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
101467Informations de copyright
Copyright © 2022 The Authors. Published by Elsevier GmbH.. All rights reserved.