Plastid ribosome protein L5 is essential for post-globular embryo development in Arabidopsis thaliana.
Arabidopsis thaliana
Embryo development
Localization
Plastid
Ribosome
Journal
Plant reproduction
ISSN: 2194-7961
Titre abrégé: Plant Reprod
Pays: Germany
ID NLM: 101602701
Informations de publication
Date de publication:
09 2022
09 2022
Historique:
received:
17
10
2021
accepted:
14
02
2022
pubmed:
6
3
2022
medline:
9
8
2022
entrez:
5
3
2022
Statut:
ppublish
Résumé
Plastid ribosomal proteins (PRPs) can play essential roles in plastid ribosome functioning that affect plant function and development. However, the roles of many PRPs remain unknown, including elucidation of which PRPs are essential or display redundancy. Here, we report that the nuclear-encoded PLASTID RIBOSOMAL PROTEIN L5 (PRPL5) is essential for early embryo development in A. thaliana, as homozygous loss-of-function mutations in the PRPL5 gene impairs chloroplast development and leads to embryo failure to develop past the globular stage. We confirmed the prpl5 embryo-lethal phenotype by generating a mutant CRISPR/Cas9 line and by genetic complementation. As PRPL5 underwent transfer to the nuclear genome early in the evolution of Embryophyta, PRPL5 can be expected to have acquired a chloroplast transit peptide. We identify and validate the presence of an N-terminal chloroplast transit peptide, but unexpectedly also confirm the presence of a conserved and functional Nuclear Localization Signal on the protein C-terminal end. This study highlights the fundamental role of the plastid translation machinery during the early stages of embryo development in plants and raises the possibility of additional roles of plastid ribosomal proteins in the nucleus.
Identifiants
pubmed: 35247095
doi: 10.1007/s00497-022-00440-9
pii: 10.1007/s00497-022-00440-9
pmc: PMC9352626
doi:
Substances chimiques
Arabidopsis Proteins
0
Ribosomal Proteins
0
ribosomal protein L5
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
189-204Informations de copyright
© 2022. The Author(s).
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