Stability of the H-cluster under whole-cell conditions-formation of an H

Biophysics Electron paramagnetic resonance (EPR) Enzyme mechanism Hydrogenase Metalloenzymes

Journal

Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry
ISSN: 1432-1327
Titre abrégé: J Biol Inorg Chem
Pays: Germany
ID NLM: 9616326

Informations de publication

Date de publication:
04 2022
Historique:
received: 20 09 2021
accepted: 03 01 2022
pubmed: 9 3 2022
medline: 12 4 2022
entrez: 8 3 2022
Statut: ppublish

Résumé

Hydrogenases are metalloenzymes that catalyze the reversible oxidation of molecular hydrogen into protons and electrons. For this purpose, [FeFe]-hydrogenases utilize a hexanuclear iron cofactor, the H-cluster. This biologically unique cofactor provides the enzyme with outstanding catalytic activities, but it is also highly oxygen sensitive. Under in vitro conditions, oxygen stable forms of the H-cluster denoted H

Identifiants

pubmed: 35258679
doi: 10.1007/s00775-022-01928-5
pii: 10.1007/s00775-022-01928-5
pmc: PMC8960641
doi:

Substances chimiques

Iron-Sulfur Proteins 0
Protons 0
Sulfides 0
Hydrogen 7YNJ3PO35Z
Hydrogenase EC 1.12.7.2
Oxygen S88TT14065

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

345-355

Subventions

Organisme : Deutsche Forschungsgemeinschaft
ID : SPP1927
Organisme : H2020 European Research Council
ID : 714102
Organisme : H2020 European Research Council
ID : 819580
Organisme : FP7 Ideas: European Research Council
ID : 765376

Informations de copyright

© 2022. The Author(s).

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Auteurs

Marco Lorenzi (M)

Molecular Biomimetics, Department of Chemistry-Ångström Laboratory, Uppsala University, Box 523, 75120, Uppsala, Sweden.

Pierre Ceccaldi (P)

Molecular Biomimetics, Department of Chemistry-Ångström Laboratory, Uppsala University, Box 523, 75120, Uppsala, Sweden.
Current Address: R&I Consultant, Home Office, Marseille, France.

Patricia Rodríguez-Maciá (P)

Department of Inorganic Spectroscopy, Max Planck Institute for Chemical Energy Conversion, Stiftstrasse 34-36, 45470, Mülheim an der Ruhr, Germany.
Current address: Department of Chemistry, Inorganic Chemistry Laboratory, University of Oxford, South Parks Road, Oxford, OX1 3QR, UK.

Holly Jayne Redman (HJ)

Molecular Biomimetics, Department of Chemistry-Ångström Laboratory, Uppsala University, Box 523, 75120, Uppsala, Sweden.

Afridi Zamader (A)

Molecular Biomimetics, Department of Chemistry-Ångström Laboratory, Uppsala University, Box 523, 75120, Uppsala, Sweden.
Laboratoire de Chimie et Biologie des Métaux, Université Grenoble Alpes, CNRS, CEA, 17 rue des Martyrs, 38054, Grenoble, France.

James A Birrell (JA)

Department of Inorganic Spectroscopy, Max Planck Institute for Chemical Energy Conversion, Stiftstrasse 34-36, 45470, Mülheim an der Ruhr, Germany.

Livia S Mészáros (LS)

Molecular Biomimetics, Department of Chemistry-Ångström Laboratory, Uppsala University, Box 523, 75120, Uppsala, Sweden.

Gustav Berggren (G)

Molecular Biomimetics, Department of Chemistry-Ångström Laboratory, Uppsala University, Box 523, 75120, Uppsala, Sweden. Gustav.Berggren@kemi.uu.se.

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Classifications MeSH