Raman spectroscopy enables highly accurate differentiation between young male and female hemp plants.
Chemometrics
HPLC
Hemp
Lutein
Raman spectroscopy
Sex determination
Journal
Planta
ISSN: 1432-2048
Titre abrégé: Planta
Pays: Germany
ID NLM: 1250576
Informations de publication
Date de publication:
13 Mar 2022
13 Mar 2022
Historique:
received:
08
02
2022
accepted:
01
03
2022
entrez:
13
3
2022
pubmed:
14
3
2022
medline:
16
3
2022
Statut:
epublish
Résumé
Hand-held Raman spectroscopy can be used for highly accurate differentiation between young male and female hemp plants. This differentiation is based on significantly different concentration of lutein in these plants. Last year, a global market of only industrial hemp attained the value of USD 4.7 billion. It is by far the fastest growing market with projected growth of 22.5% between 2021 and 2026. Hemp (Cannabis sativa L.) is a dioecious species that has separate male and female plants. In hemp farming, female plants are strongly preferred because male plants do not produce sufficient amount of cannabinoids. Male plants are also eliminated to minimize a possibility of uncontrolled cross-fertilization of plants. Silver treatments can induce development of male flowers on genetically female plants in order to produce feminized seed. Resulting cannabinoid hemp production fields should contain 100% female plants. However, any unintended pollination from male plants can produce unwanted males in production fields. Therefore, there is a growing demand for a label-free, non-invasive, and confirmatory approach that can be used to differentiate between male and female plants before flowering. In this study, we examined the extent to which Raman spectroscopy, an emerging optical technique, can be used for the accurate differentiation between young male and female hemp plants. Our findings show that Raman spectroscopy enables differentiation between male and female plants with 90% and 94% accuracy on the level of young and mature plants, respectively. Such analysis is entirely non-invasive and non-destructive to plants and can be performed in seconds using a hand-held spectrometer. High-performance liquid chromatography (HPLC) analysis and collected Raman spectra demonstrate that this spectroscopic differentiation is based on significantly different concentrations of carotenoids in male vs female plants. These findings open up a new avenue for quality control of plants grown in both field and a greenhouse.
Identifiants
pubmed: 35279786
doi: 10.1007/s00425-022-03865-8
pii: 10.1007/s00425-022-03865-8
doi:
Substances chimiques
Cannabinoids
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
85Informations de copyright
© 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.
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