Targeted introduction of heritable point mutations into the plant mitochondrial genome.
Journal
Nature plants
ISSN: 2055-0278
Titre abrégé: Nat Plants
Pays: England
ID NLM: 101651677
Informations de publication
Date de publication:
03 2022
03 2022
Historique:
received:
26
08
2021
accepted:
10
02
2022
pubmed:
19
3
2022
medline:
31
3
2022
entrez:
18
3
2022
Statut:
ppublish
Résumé
The development of technologies for the genetic manipulation of mitochondrial genomes remains a major challenge. Here we report a method for the targeted introduction of mutations into plant mitochondrial DNA (mtDNA) that we refer to as transcription activator-like effector nuclease (TALEN) gene-drive mutagenesis (GDM), or TALEN-GDM. The method combines TALEN-induced site-specific cleavage of the mtDNA with selection for mutations that confer resistance to the TALEN cut. Applying TALEN-GDM to the tobacco mitochondrial nad9 gene, we isolated a large set of mutants carrying single amino acid substitutions in the Nad9 protein. The mutants could be purified to homochondriomy and stably inherited their edited mtDNA in the expected maternal fashion. TALEN-GDM induces both transitions and transversions, and can access most nucleotide positions within the TALEN binding site. Our work provides an efficient method for targeted mitochondrial genome editing that produces genetically stable, homochondriomic and fertile plants with specific point mutations in their mtDNA.
Identifiants
pubmed: 35301443
doi: 10.1038/s41477-022-01108-y
pii: 10.1038/s41477-022-01108-y
pmc: PMC8940627
mid: EMS142185
doi:
Substances chimiques
DNA, Plant
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
245-256Subventions
Organisme : European Research Council
ID : 669982
Pays : International
Informations de copyright
© 2022. The Author(s).
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