Biochemical and structural characterization of an aromatic ring-hydroxylating dioxygenase for terephthalic acid catabolism.

Poly(ethylene terephthalate) Rieske dioxygenase oxygenase plastic terephthalate

Journal

Proceedings of the National Academy of Sciences of the United States of America
ISSN: 1091-6490
Titre abrégé: Proc Natl Acad Sci U S A
Pays: United States
ID NLM: 7505876

Informations de publication

Date de publication:
29 03 2022
Historique:
entrez: 21 3 2022
pubmed: 22 3 2022
medline: 4 5 2022
Statut: ppublish

Résumé

SignificanceMore than 400 million tons of plastic waste is produced each year, the overwhelming majority of which ends up in landfills. Bioconversion strategies aimed at plastics have emerged as important components of enabling a circular economy for synthetic plastics, especially those that exhibit chemically similar linkages to those found in nature, such as polyesters. The enzyme system described in this work is essential for mineralization of the xenobiotic components of poly(ethylene terephthalate) (PET) in the biosphere. Our description of its structure and substrate preferences lays the groundwork for in vivo or ex vivo engineering of this system for PET upcycling.

Identifiants

pubmed: 35312352
doi: 10.1073/pnas.2121426119
pmc: PMC9060491
doi:

Substances chimiques

Phthalic Acids 0
Plastics 0
Polyethylene Terephthalates 0
terephthalic acid 6S7NKZ40BQ
Dioxygenases EC 1.13.11.-

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

e2121426119

Subventions

Organisme : NIGMS NIH HHS
ID : R35 GM136390
Pays : United States

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Auteurs

William M Kincannon (WM)

Department of Biochemistry, Montana State University, Bozeman, MT 59717.

Michael Zahn (M)

Centre for Enzyme Innovation, University of Portsmouth, Portsmouth, PO1 2PY, United Kingdom.
Bio-Optimized Technologies to Keep Thermoplastics out of Landfills and the Environment Consortium, Golden, CO 80401.

Rita Clare (R)

Department of Biochemistry, Montana State University, Bozeman, MT 59717.
Bio-Optimized Technologies to Keep Thermoplastics out of Landfills and the Environment Consortium, Golden, CO 80401.

Jessica Lusty Beech (J)

Department of Biochemistry, Montana State University, Bozeman, MT 59717.
Bio-Optimized Technologies to Keep Thermoplastics out of Landfills and the Environment Consortium, Golden, CO 80401.

Ari Romberg (A)

Department of Biochemistry, Montana State University, Bozeman, MT 59717.

James Larson (J)

Department of Biochemistry, Montana State University, Bozeman, MT 59717.

Brian Bothner (B)

Department of Biochemistry, Montana State University, Bozeman, MT 59717.

Gregg T Beckham (GT)

Bio-Optimized Technologies to Keep Thermoplastics out of Landfills and the Environment Consortium, Golden, CO 80401.
National Renewable Energy Laboratory, Renewable Resources and Enabling Sciences Center, Golden, CO 80401.

John E McGeehan (JE)

Centre for Enzyme Innovation, University of Portsmouth, Portsmouth, PO1 2PY, United Kingdom.
Bio-Optimized Technologies to Keep Thermoplastics out of Landfills and the Environment Consortium, Golden, CO 80401.

Jennifer L DuBois (JL)

Department of Biochemistry, Montana State University, Bozeman, MT 59717.
Bio-Optimized Technologies to Keep Thermoplastics out of Landfills and the Environment Consortium, Golden, CO 80401.

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Classifications MeSH