Silencing of Ago-2 Interacting Protein SERBP1 Relieves KCC2 Repression by miR-92 in Neurons.
Ago2
KCC2
RNA-binding protein
RNA-induced silencing complex (RISC)
SERBP1
microRNA
Journal
Cells
ISSN: 2073-4409
Titre abrégé: Cells
Pays: Switzerland
ID NLM: 101600052
Informations de publication
Date de publication:
20 03 2022
20 03 2022
Historique:
received:
18
02
2022
revised:
11
03
2022
accepted:
18
03
2022
entrez:
25
3
2022
pubmed:
26
3
2022
medline:
13
4
2022
Statut:
epublish
Résumé
RNA-binding proteins (RBPs) play important roles in modulating miRNA-mediated mRNA target repression. Argonaute2 (Ago2) is an essential component of the RNA-induced silencing complex (RISC) that plays a central role in silencing mechanisms via small non-coding RNA molecules known as siRNAs and miRNAs. Small RNAs loaded into Argonaute proteins catalyze endoribonucleolytic cleavage of target RNAs or recruit factors responsible for translational silencing and mRNA target destabilization. In previous studies we have shown that KCC2, a neuronal Cl (-) extruding K (+) Cl (-) co-transporter 2, is regulated by miR-92 in neuronal cells. Searching for Ago2 partners by immunoprecipitation and LC-MS/MS analysis, we isolated among other proteins the Serpine mRNA binding protein 1 (SERBP1) from SH-SY5Y neuroblastoma cells. Exploring the role of SERBP1 in miRNA-mediated gene silencing in SH-SY5Y cells and primary hippocampal neurons, we demonstrated that SERBP1 silencing regulates KCC2 expression through the 3' untranslated region (UTR). In addition, we found that SERBP1 as well as Ago2/miR-92 complex bind to KCC2 3'UTR. Finally, we demonstrated the attenuation of miR-92-mediated repression of KCC2 3'UTR by SERBP1 silencing. These findings advance our knowledge regarding the miR-92-mediated modulation of KCC2 translation in neuronal cells and highlight SERBP1 as a key component of this gene regulation.
Identifiants
pubmed: 35326503
pii: cells11061052
doi: 10.3390/cells11061052
pmc: PMC8947033
pii:
doi:
Substances chimiques
3' Untranslated Regions
0
MicroRNAs
0
RNA, Messenger
0
RNA-Induced Silencing Complex
0
Symporters
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Sapienza University of Rome
ID : RM11916B410542D4
Organisme : National Research Council
ID : DSB.AD009.001.004
Références
Front Cell Dev Biol. 2020 Jun 03;8:409
pubmed: 32582699
Cell Rep. 2016 May 24;15(8):1634-47
pubmed: 27184842
FEBS Lett. 2003 Jan 2;533(1-3):14-20
pubmed: 12505151
Int J Mol Sci. 2020 Jan 14;21(2):
pubmed: 31947619
Nature. 2011 Feb 10;470(7333):284-8
pubmed: 21307942
Nature. 1999 Jan 21;397(6716):251-5
pubmed: 9930699
Hippocampus. 2014 Dec;24(12):1458-65
pubmed: 24990518
Proc Natl Acad Sci U S A. 2016 Mar 15;113(11):E1545-54
pubmed: 26929374
J Neurochem. 2010 May;113(3):591-600
pubmed: 20050974
Cell. 2007 Dec 28;131(7):1273-86
pubmed: 18155131
Cell Biochem Funct. 2018 Aug;36(6):312-322
pubmed: 30039520
J Cell Biochem. 2012 Aug;113(8):2721-8
pubmed: 22442049
RNA. 2013 Nov;19(11):1583-93
pubmed: 24049110
EMBO J. 2018 Jun 1;37(11):
pubmed: 29712715
Nat Rev Mol Cell Biol. 2019 Jan;20(1):5-20
pubmed: 30228348
Commun Integr Biol. 2010 Nov;3(6):499-503
pubmed: 21331224
Mol Biol Cell. 2010 May 1;21(9):1462-9
pubmed: 20237157
J Biol Chem. 2001 Feb 2;276(5):3341-7
pubmed: 11001948
EMBO J. 2015 Sep 2;34(17):2237-54
pubmed: 26105073
Br J Cancer. 2012 Jan 17;106(2):366-74
pubmed: 22108519
PLoS One. 2013 May 28;8(5):e63862
pubmed: 23724009
Mol Cell. 2018 Feb 1;69(3):517-532.e11
pubmed: 29395067
J Biochem. 2011 Dec;150(6):687-94
pubmed: 21878476
Curr Biol. 2005 Dec 6;15(23):2149-55
pubmed: 16289642
J Proteome Res. 2015 Nov 6;14(11):4571-80
pubmed: 26398198
Nat Cell Biol. 2009 Dec;11(12):1411-20
pubmed: 19898466
Genome Biol. 2020 Aug 6;21(1):195
pubmed: 32762776
Nat Rev Neurosci. 2002 Sep;3(9):728-39
pubmed: 12209121
Trends Neurosci. 2021 May;44(5):378-392
pubmed: 33640193
Exp Neurobiol. 2020 Apr 30;29(2):138-149
pubmed: 32408404
Acta Neuropathol Commun. 2019 Dec 3;7(1):196
pubmed: 31796123
Nat Protoc. 2006;1(1):302-7
pubmed: 17406249
Mol Cell. 2011 Jun 10;42(5):673-88
pubmed: 21658607
Nature. 2021 Jul;595(7865):120-124
pubmed: 34079125
Nat Commun. 2017 Jun 06;8:15690
pubmed: 28585547
Nat Commun. 2019 Feb 19;10(1):844
pubmed: 30783097
RNA Biol. 2015;12(9):922-6
pubmed: 26274611
World J Biol Chem. 2019 Nov 21;10(3):44-64
pubmed: 31768228
Mol Cell Proteomics. 2012 Nov;11(11):1442-56
pubmed: 22918229
Front Mol Biosci. 2020 Jan 31;7:8
pubmed: 32118035
Philos Trans R Soc Lond B Biol Sci. 2018 Jan 19;373(1738):
pubmed: 29203717
Crit Rev Biochem Mol Biol. 2020 Feb;55(1):33-53
pubmed: 32164444
Proc Natl Acad Sci U S A. 2008 Oct 7;105(40):15281-6
pubmed: 18829439
Mol Biol Cell. 2009 Jul;20(14):3273-84
pubmed: 19458189
Neurobiol Aging. 2009 Dec;30(12):1962-74
pubmed: 18378046
PLoS One. 2013 Jul 22;8(7):e69764
pubmed: 23894536
EMBO Rep. 2014 May;15(5):548-56
pubmed: 24723684
Nat Rev Mol Cell Biol. 2018 May;19(5):327-341
pubmed: 29339797
Nat Commun. 2021 Aug 20;12(1):5057
pubmed: 34417449
Nucleic Acids Res. 2015 Jul 27;43(13):6321-33
pubmed: 26068472
FEBS J. 2014 Jan;281(1):352-64
pubmed: 24205981
Nat Rev Cancer. 2011 Aug 05;11(9):644-56
pubmed: 21822212
Cells. 2020 Jan 17;9(1):
pubmed: 31963584
FEBS Lett. 2011 Oct 3;585(19):2965-71
pubmed: 21846468
RNA Biol. 2021 Sep;18(9):1252-1264
pubmed: 33030396