HydroZitLa inhibits calcium oxalate stone formation in nephrolithic rats and promotes longevity in nematode Caenorhabditis elegans.


Journal

Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288

Informations de publication

Date de publication:
24 03 2022
Historique:
received: 07 06 2021
accepted: 22 02 2022
entrez: 25 3 2022
pubmed: 26 3 2022
medline: 6 5 2022
Statut: epublish

Résumé

Low fluid intake, low urinary citrate excretion, and high oxidative stress are main causative factors of calcium oxalate (CaOx) nephrolithiasis. HydroZitLa contains citrate and natural antioxidants and is developed to correct these three factors simultaneously. Antioxidants theoretically can prolong the lifespan of organisms. In this study, we preclinically investigated the antilithogenic, lifespan-extending and anti-aging effects of HydroZitLa in HK-2 cells, male Wistar rats, and Caenorhabditis elegans. HydroZitLa significantly inhibited CaOx crystal aggregation in vitro and reduced oxidative stress in HK-2 cells challenged with lithogenic factors. For experimental nephrolithiasis, rats were divided into four groups: ethylene glycol (EG), EG + HydroZitLa, EG + Uralyt-U, and untreated control. CaOx deposits in kidneys of EG + HydroZitLa and EG + Uralyt-U rats were significantly lower than those of EG rats. Intrarenal expression of 4-hydroxynonenal in EG + HydroZitLa rats was significantly lower than that of EG rats. The urinary oxalate levels of EG + HydroZitLa and EG + Uralyt-U rats were significantly lower than those of EG rats. The urinary citrate levels of EG + HydroZitLa and EG + Uralyt-U rats were restored to the level in normal control rats. In C. elegans, HydroZitLa supplementation significantly extended the median lifespan of nematodes up to 34% without altering feeding ability. Lipofuscin accumulation in HydroZitLa-supplemented nematodes was significantly lower than that of non-supplemented control. Additionally, HydroZitLa inhibited telomere shortening, p16 upregulation, and premature senescence in HK-2 cells exposed to lithogenic stressors. Conclusions, HydroZitLa inhibited oxidative stress and CaOx formation both in vitro and in vivo. HydroZitLa extended the lifespan and delayed the onset of aging in C. elegans and human kidney cells. This preclinical evidence suggests that HydroZitLa is beneficial for inhibiting CaOx stone formation, promoting longevity, and slowing down aging.

Identifiants

pubmed: 35332173
doi: 10.1038/s41598-022-08316-8
pii: 10.1038/s41598-022-08316-8
pmc: PMC8948263
doi:

Substances chimiques

Antioxidants 0
Calcium Oxalate 2612HC57YE
Citric Acid 2968PHW8QP
Ethylene Glycol FC72KVT52F

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

5102

Informations de copyright

© 2022. The Author(s).

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Auteurs

Nalinthip Lordumrongkiat (N)

Departments of Biochemistry, Faculty of Medicine, Chulalongkorn University, Rama IV Rd, Bangkok, 10330, Thailand.

Nattida Chotechuang (N)

Department of Food Technology, Faculty of Science, Chulalongkorn University, Bangkok, Thailand.

Mani Iyer Prasanth (MI)

Natural Products for Neuroprotection and Anti-Ageing Research Unit, Department of Clinical Chemistry, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok, Thailand.

Depicha Jindatip (D)

Departments of Anatomy, Faculty of Medicine, Chulalongkorn University, Bangkok, 10330, Thailand.

Chakriwong Ma-On (C)

Department of Pathology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.

Kamonchanok Chuenwisad (K)

Departments of Biochemistry, Faculty of Medicine, Chulalongkorn University, Rama IV Rd, Bangkok, 10330, Thailand.

Asada Leelahavanichkul (A)

Departments of Microbiology, Faculty of Medicine, Chulalongkorn University, Bangkok, 10330, Thailand.

Tewin Tencomnao (T)

Natural Products for Neuroprotection and Anti-Ageing Research Unit, Department of Clinical Chemistry, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok, Thailand.

Chanchai Boonla (C)

Departments of Biochemistry, Faculty of Medicine, Chulalongkorn University, Rama IV Rd, Bangkok, 10330, Thailand. chanchai.b@chula.ac.th.

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