Aegilops sharonensis genome-assisted identification of stem rust resistance gene Sr62.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
25 03 2022
25 03 2022
Historique:
received:
09
07
2021
accepted:
24
02
2022
entrez:
26
3
2022
pubmed:
27
3
2022
medline:
14
4
2022
Statut:
epublish
Résumé
The wild relatives and progenitors of wheat have been widely used as sources of disease resistance (R) genes. Molecular identification and characterization of these R genes facilitates their manipulation and tracking in breeding programmes. Here, we develop a reference-quality genome assembly of the wild diploid wheat relative Aegilops sharonensis and use positional mapping, mutagenesis, RNA-Seq and transgenesis to identify the stem rust resistance gene Sr62, which has also been transferred to common wheat. This gene encodes a tandem kinase, homologues of which exist across multiple taxa in the plant kingdom. Stable Sr62 transgenic wheat lines show high levels of resistance against diverse isolates of the stem rust pathogen, highlighting the utility of Sr62 for deployment as part of a polygenic stack to maximize the durability of stem rust resistance.
Identifiants
pubmed: 35338132
doi: 10.1038/s41467-022-29132-8
pii: 10.1038/s41467-022-29132-8
pmc: PMC8956640
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1607Subventions
Organisme : Biotechnology and Biological Sciences Research Council
ID : BBS/E/J/000PR9780
Pays : United Kingdom
Informations de copyright
© 2022. The Author(s).
Références
Bioinformatics. 2009 Jul 15;25(14):1754-60
pubmed: 19451168
Plant J. 2016 Nov;88(3):452-467
pubmed: 27402341
Nat Biotechnol. 2022 Mar;40(3):422-431
pubmed: 34725503
Nat Biotechnol. 2016 Jun;34(6):652-5
pubmed: 27111722
Nucleic Acids Res. 2021 Jan 8;49(D1):D1452-D1463
pubmed: 33170273
Commun Biol. 2018 Feb 8;1:13
pubmed: 30271900
Plant Genome. 2017 Nov;10(3):
pubmed: 29293809
Proc Natl Acad Sci U S A. 2017 Nov 7;114(45):E9483-E9492
pubmed: 29078294
Chromosoma. 2003 May;111(8):509-17
pubmed: 12684822
Plant Cell. 2018 Feb;30(2):285-299
pubmed: 29382771
PLoS One. 2017 Oct 26;12(10):e0185056
pubmed: 29073143
Nat Genet. 2015 Dec;47(12):1494-8
pubmed: 26551671
Nat Commun. 2022 Mar 25;13(1):1607
pubmed: 35338132
Philos Trans R Soc Lond B Biol Sci. 2012 Sep 19;367(1602):2619-39
pubmed: 22889912
Trends Biochem Sci. 1998 Dec;23(12):454-6
pubmed: 9868361
Methods Mol Biol. 2017;1659:207-213
pubmed: 28856653
Nat Protoc. 2015 Jun;10(6):845-58
pubmed: 25950237
Nat Biotechnol. 2021 Jun;39(6):652
pubmed: 34113041
Cell. 2005 Jun 3;121(5):749-59
pubmed: 15935761
Science. 1988 Jul 1;241(4861):42-52
pubmed: 3291115
Mol Plant. 2018 Jun 4;11(6):874-878
pubmed: 29567451
Plant Physiol. 2019 Apr;179(4):1362-1372
pubmed: 30593453
Gigascience. 2012 Dec 27;1(1):18
pubmed: 23587118
Annu Rev Genet. 2017 Nov 27;51:435-454
pubmed: 28934591
Genome Biol. 2016 Oct 31;17(1):221
pubmed: 27795210
Nucleic Acids Res. 2007 Jan;35(Database issue):D883-7
pubmed: 17145706
Plant Cell. 2004 Oct;16(10):2809-21
pubmed: 15367718
Plant Methods. 2008 Sep 26;4:22
pubmed: 18822125
New Phytol. 2018 Jul;219(1):17-24
pubmed: 29131341
Genetics. 2000 Dec;156(4):2033-41
pubmed: 11102393
Plant Genome. 2017 Jul;10(2):
pubmed: 28724065
Sci Data. 2017 Apr 27;4:170044
pubmed: 28448065
Nat Biotechnol. 2019 Feb;37(2):139-143
pubmed: 30718880
PLoS One. 2011 May 04;6(5):e19379
pubmed: 21573248
Science. 1993 Nov 26;262(5138):1432-6
pubmed: 7902614
Nat Immunol. 2006 Dec;7(12):1243-9
pubmed: 17110940
Plant Dis. 2007 Aug;91(8):942-950
pubmed: 30780426
Nature. 2018 May;557(7705):424-428
pubmed: 29743678
Nat Biotechnol. 2019 Aug;37(8):907-915
pubmed: 31375807
Proc Natl Acad Sci U S A. 2002 Jul 9;99(14):9328-33
pubmed: 12077318
J Mol Biol. 2009 Jul 17;390(3):560-77
pubmed: 19447117
PLoS Genet. 2019 Jan 18;15(1):e1007819
pubmed: 30657772
DNA Res. 2019 Apr 1;26(2):171-182
pubmed: 30715317
Genome Biol. 2015 Jan 31;16:26
pubmed: 25637298
Science. 2009 Mar 6;323(5919):1360-3
pubmed: 19229000
Genome. 2014 Jun;57(6):309-16
pubmed: 25209724
Plant J. 2022 Apr;110(1):179-192
pubmed: 34997796
Cell. 2002 Mar 22;108(6):743-54
pubmed: 11955429
Genome Biol. 2019 Dec 18;20(1):284
pubmed: 31849336
Nature. 2012 Nov 29;491(7426):711-6
pubmed: 23075845
Acta Crystallogr D Biol Crystallogr. 2011 Apr;67(Pt 4):386-94
pubmed: 21460457
Science. 2014 Jul 18;345(6194):1250092
pubmed: 25035499
J Mol Biol. 1970 Mar;48(3):443-53
pubmed: 5420325
Nucleic Acids Res. 2012 Jul;40(Web Server issue):W452-7
pubmed: 22689647
Nat Commun. 2020 Feb 3;11(1):680
pubmed: 32015344
Science. 2018 Aug 3;361(6401):451-452
pubmed: 30072526
Nat Commun. 2018 Oct 3;9(1):3735
pubmed: 30282993
Plant Dis. 2014 Oct;98(10):1309-1320
pubmed: 30703930
Science. 2013 Aug 16;341(6147):783-786
pubmed: 23811222
Nat Biotechnol. 2017 Aug;35(8):793-796
pubmed: 28504667
Theor Appl Genet. 2015 Jun;128(6):1049-59
pubmed: 25748115
Nat Genet. 2019 May;51(5):885-895
pubmed: 30962619
Genome. 2007 Nov;50(11):1029-37
pubmed: 18059548
Theor Appl Genet. 2017 Jun;130(6):1207-1222
pubmed: 28275817
Science. 2018 Aug 17;361(6403):
pubmed: 30115783
Science. 2013 Aug 16;341(6147):786-8
pubmed: 23811228
BMC Genomics. 2008 Jun 19;9:294
pubmed: 18565235
Plant Cell. 2008 Aug;20(8):2009-17
pubmed: 18723576
Nucleic Acids Res. 2020 Jan 8;48(D1):D689-D695
pubmed: 31598706
Cell Host Microbe. 2015 Sep 9;18(3):285-95
pubmed: 26355215
Bioinformatics. 2015 Sep 1;31(17):2885-7
pubmed: 25953801
Plant Dis. 2000 Feb;84(2):203
pubmed: 30841334
Plant Commun. 2019 Dec 18;1(1):100016
pubmed: 33404540
Phytopathology. 2015 Jul;105(7):917-28
pubmed: 25775107
Plant Cell. 2006 Oct;18(10):2792-806
pubmed: 17028203
Nature. 2017 Apr 26;544(7651):427-433
pubmed: 28447635
Mol Plant. 2021 Jul 5;14(7):1053-1070
pubmed: 33991673
Nature. 2017 Nov 23;551(7681):498-502
pubmed: 29143815
Genetics. 2005 Jun;170(2):823-9
pubmed: 15802508
Proc Natl Acad Sci U S A. 2008 Jul 15;105(28):9691-6
pubmed: 18599450
New Phytol. 2020 Jan;225(2):948-959
pubmed: 31487050
Cell. 2009 May 15;137(4):773-83
pubmed: 19450522
Nature. 2017 Feb 2;542(7640):145-146
pubmed: 28179687
Genome. 1995 Apr;38(2):283-9
pubmed: 18470167
Nature. 2006 Nov 16;444(7117):323-9
pubmed: 17108957
PLoS One. 2013;8(2):e57994
pubmed: 23469124
Nat Plants. 2015 Nov 30;1:15186
pubmed: 27251721
Genome. 1996 Apr;39(2):293-306
pubmed: 18469894
Bioinformatics. 2009 Aug 15;25(16):2078-9
pubmed: 19505943
Nat Commun. 2021 Jun 7;12(1):3378
pubmed: 34099713
Plant Dis. 2008 Jun;92(6):923-926
pubmed: 30769714
Plant Physiol. 2009 May;150(1):12-26
pubmed: 19321712
Plant Dis. 2009 Apr;93(4):367-370
pubmed: 30764215
Plant Methods. 2019 Oct 26;15:121
pubmed: 31673278
Bioinformatics. 2016 Jun 15;32(12):i201-i208
pubmed: 27307618
Mol Plant Microbe Interact. 2021 Oct;34(10):1094-1102
pubmed: 34096764
PLoS Genet. 2018 Apr 3;14(4):e1007287
pubmed: 29614079
Plant J. 2013 Nov;76(3):494-505
pubmed: 23889683
Plant Physiol. 2020 Jun;183(2):468-482
pubmed: 32184345