Membrane thickness, lipid phase and sterol type are determining factors in the permeability of membranes to small solutes.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
25 03 2022
25 03 2022
Historique:
received:
09
07
2021
accepted:
02
03
2022
entrez:
26
3
2022
pubmed:
27
3
2022
medline:
14
4
2022
Statut:
epublish
Résumé
Cell membranes provide a selective semi-permeable barrier to the passive transport of molecules. This property differs greatly between organisms. While the cytoplasmic membrane of bacterial cells is highly permeable for weak acids and glycerol, yeasts can maintain large concentration gradients. Here we show that such differences can arise from the physical state of the plasma membrane. By combining stopped-flow kinetic measurements with molecular dynamics simulations, we performed a systematic analysis of the permeability of a variety of small molecules through synthetic membranes of different lipid composition to obtain detailed molecular insight into the permeation mechanisms. While membrane thickness is an important parameter for the permeability through fluid membranes, the largest differences occur when the membranes transit from the liquid-disordered to liquid-ordered and/or to gel state, which is in agreement with previous work on passive diffusion of water. By comparing our results with in vivo measurements from yeast, we conclude that the yeast membrane exists in a highly ordered and rigid state, which is comparable to synthetic saturated DPPC-sterol membranes.
Identifiants
pubmed: 35338137
doi: 10.1038/s41467-022-29272-x
pii: 10.1038/s41467-022-29272-x
pmc: PMC8956743
doi:
Substances chimiques
Lipid Bilayers
0
Sterols
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1605Informations de copyright
© 2022. The Author(s).
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