Characterization of diarrhoeagenic Escherichia coli with special reference to antimicrobial resistance isolated from hospitalized diarrhoeal patients in Kolkata (2012-2019), India.
Adolescent
Anti-Bacterial Agents
/ pharmacology
Anti-Infective Agents
/ therapeutic use
Child
Child, Preschool
Coinfection
Diarrhea
/ epidemiology
Drug Resistance, Bacterial
/ genetics
Escherichia coli
Escherichia coli Infections
/ drug therapy
Feces
/ microbiology
Humans
Microbial Sensitivity Tests
EAEC
EPEC
ETEC
antimicrobial resistance
diarrhoeagenic Escherichia coli
Journal
Journal of applied microbiology
ISSN: 1365-2672
Titre abrégé: J Appl Microbiol
Pays: England
ID NLM: 9706280
Informations de publication
Date de publication:
Jun 2022
Jun 2022
Historique:
revised:
22
03
2022
received:
19
01
2022
accepted:
24
03
2022
pubmed:
27
3
2022
medline:
21
5
2022
entrez:
26
3
2022
Statut:
ppublish
Résumé
This study analyses the prevalence and antimicrobial resistance (AMR) of major diarrhoeagenic Escherichia coli (DEC) pathotypes detected in hospitalized diarrhoeal patients in Kolkata, India, during 2012-2019. A total of 8891 stool samples were collected from the Infectious Diseases Hospital, Kolkata and screened for the presence of enteric pathogens. Multiplex PCR identified the presence of DEC in 7.8% of the samples, in which ETEC was most common (47.7%) followed by EAEC (38.4%) and EPEC (13.9%). About 54% cases were due to sole DEC infections. Majority of the mixed DEC infections were caused by the Vibrio spp. (19.1%) followed by Rotavirus (14.1%) and Campylobacter spp. (8.4%). ETEC and EAEC were associated significantly with diarrhoea in children <5 years of age, whereas EPEC and also ETEC were prevalent in patients aged between 5 and 14 years. AMR profile showed high prevalence of multidrug resistance (MDR) among DEC (56.9%) in which 9% were resistant to antibiotics of six different antimicrobial classes. Screening of the AMR conferring genes of DEC showed the presence of bla These findings highlighted the high prevalence of MDR in major DEC pathotypes that could be considered as the leading aetiological bacterial agent responsible for diarrhoea and suggests a significant public health threat. The results of this study can help to improve the understanding of the epidemiology of DEC infections in patients with diarrhoea. Monitoring of AMR surveillance needs special attention because the DEC isolates were highly resistant to commonly used antimicrobials in the treatment of diarrhoea.
Substances chimiques
Anti-Bacterial Agents
0
Anti-Infective Agents
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
4544-4554Subventions
Organisme : Japan Agency for Medical Research and Development (AMED), Japan
Organisme : Indian Council of Medical Research (ICMR), India
Organisme : Okayama University, Japan
Organisme : Council of Scientific and Industrial Research, India
Informations de copyright
© 2022 Society for Applied Microbiology.
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