Genetic and biocatalytic basis of formate dependent growth of Escherichia coli strains evolved in continuous culture.
Continuous culture
Escherichia coli
Evolution
Formate assimilation
Lipoamide dehydrogenase
Methylene-H(4)F dehydrogenase/cyclohydrolase
Mutation analysis
One carbon metabolism
Journal
Metabolic engineering
ISSN: 1096-7184
Titre abrégé: Metab Eng
Pays: Belgium
ID NLM: 9815657
Informations de publication
Date de publication:
07 2022
07 2022
Historique:
received:
20
12
2021
revised:
22
02
2022
accepted:
14
03
2022
pubmed:
29
3
2022
medline:
15
6
2022
entrez:
28
3
2022
Statut:
ppublish
Résumé
The reductive glycine pathway was described as the most energetically favorable synthetic route of aerobic formate assimilation. Here we report the successful implementation of formatotrophy in Escherichia coli by means of a stepwise adaptive evolution strategy. Medium swap and turbidostat regimes of continuous culture were applied to force the channeling of carbon flux through the synthetic pathway to pyruvate establishing growth on formate and CO
Identifiants
pubmed: 35341982
pii: S1096-7176(22)00046-5
doi: 10.1016/j.ymben.2022.03.010
pii:
doi:
Substances chimiques
Formates
0
Carbon Dioxide
142M471B3J
Glycine
TE7660XO1C
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
200-214Informations de copyright
Copyright © 2022 International Metabolic Engineering Society. Published by Elsevier Inc. All rights reserved.