Role for a Lytic Polysaccharide Monooxygenase in Cell Wall Remodeling in Streptomyces coelicolor.
LPMO
apical growth
cell wall
cell wall biosynthesis
cellulose
glycan
morphology
peptidoglycan
Journal
mBio
ISSN: 2150-7511
Titre abrégé: mBio
Pays: United States
ID NLM: 101519231
Informations de publication
Date de publication:
26 04 2022
26 04 2022
Historique:
pubmed:
1
4
2022
medline:
29
4
2022
entrez:
31
3
2022
Statut:
ppublish
Résumé
Peptidoglycan is a major constituent of the bacterial cell wall and an important determinant for providing protection to cells. In addition to peptidoglycan, many bacteria synthesize other glycans that become part of the cell wall. Streptomycetes grow apically, where they synthesize a glycan that is exposed at the outer surface, but how it gets there is unknown. Here, we show that deposition of the apical glycan at the cell surface of Streptomyces coelicolor depends on two key enzymes, the glucanase CslZ and the lytic polysaccharide monooxygenase LpmP. Activity of these enzymes allows localized remodeling and degradation of the peptidoglycan, and we propose that this facilitates passage of the glycan. The absence of both enzymes not only prevents morphological development but also sensitizes strains to lysozyme. Given that lytic polysaccharide monooxygenases are commonly found in microbes, this newly identified biological role in cell wall remodeling may be widespread.
Identifiants
pubmed: 35357207
doi: 10.1128/mbio.00456-22
pmc: PMC9040799
doi:
Substances chimiques
Dietary Carbohydrates
0
Peptidoglycan
0
Polysaccharides
0
Mixed Function Oxygenases
EC 1.-
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0045622Références
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