Segmental duplications and their variation in a complete human genome.


Journal

Science (New York, N.Y.)
ISSN: 1095-9203
Titre abrégé: Science
Pays: United States
ID NLM: 0404511

Informations de publication

Date de publication:
04 2022
Historique:
entrez: 31 3 2022
pubmed: 1 4 2022
medline: 6 4 2022
Statut: ppublish

Résumé

Despite their importance in disease and evolution, highly identical segmental duplications (SDs) are among the last regions of the human reference genome (GRCh38) to be fully sequenced. Using a complete telomere-to-telomere human genome (T2T-CHM13), we present a comprehensive view of human SD organization. SDs account for nearly one-third of the additional sequence, increasing the genome-wide estimate from 5.4 to 7.0% [218 million base pairs (Mbp)]. An analysis of 268 human genomes shows that 91% of the previously unresolved T2T-CHM13 SD sequence (68.3 Mbp) better represents human copy number variation. Comparing long-read assemblies from human (

Identifiants

pubmed: 35357917
doi: 10.1126/science.abj6965
pmc: PMC8979283
mid: NIHMS1764181
doi:

Substances chimiques

GTPase-Activating Proteins 0
Proto-Oncogene Proteins 0
TBC1D3 protein, human 0

Types de publication

Journal Article Research Support, N.I.H., Extramural

Langues

eng

Sous-ensembles de citation

IM

Pagination

eabj6965

Subventions

Organisme : NIGMS NIH HHS
ID : T32 GM007445
Pays : United States
Organisme : NHGRI NIH HHS
ID : U01 HG010973
Pays : United States
Organisme : NHGRI NIH HHS
ID : U41 HG007234
Pays : United States
Organisme : NHGRI NIH HHS
ID : U24 HG007234
Pays : United States
Organisme : NHGRI NIH HHS
ID : R01 HG011274
Pays : United States
Organisme : NHGRI NIH HHS
ID : U01 HG010971
Pays : United States
Organisme : NHGRI NIH HHS
ID : R01 HG009190
Pays : United States
Organisme : NHGRI NIH HHS
ID : R01 HG002385
Pays : United States

Commentaires et corrections

Type : CommentIn

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Auteurs

Mitchell R Vollger (MR)

Department of Genome Sciences, University of Washington School of Medicine, Seattle, WA, USA.

Xavi Guitart (X)

Department of Genome Sciences, University of Washington School of Medicine, Seattle, WA, USA.

Philip C Dishuck (PC)

Department of Genome Sciences, University of Washington School of Medicine, Seattle, WA, USA.

Ludovica Mercuri (L)

Department of Biology, University of Bari, Aldo Moro, Bari 70125, Italy.

William T Harvey (WT)

Department of Genome Sciences, University of Washington School of Medicine, Seattle, WA, USA.

Ariel Gershman (A)

Department of Molecular Biology and Genetics, Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD, USA.

Mark Diekhans (M)

UC Santa Cruz Genomics Institute, University of California Santa Cruz, Santa Cruz, CA, USA.

Arvis Sulovari (A)

Department of Genome Sciences, University of Washington School of Medicine, Seattle, WA, USA.

Katherine M Munson (KM)

Department of Genome Sciences, University of Washington School of Medicine, Seattle, WA, USA.

Alexandra P Lewis (AP)

Department of Genome Sciences, University of Washington School of Medicine, Seattle, WA, USA.

Kendra Hoekzema (K)

Department of Genome Sciences, University of Washington School of Medicine, Seattle, WA, USA.

David Porubsky (D)

Department of Genome Sciences, University of Washington School of Medicine, Seattle, WA, USA.

Ruiyang Li (R)

Department of Genome Sciences, University of Washington School of Medicine, Seattle, WA, USA.

Sergey Nurk (S)

Genome Informatics Section, Computational and Statistical Genomics Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, MD, USA.

Sergey Koren (S)

Genome Informatics Section, Computational and Statistical Genomics Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, MD, USA.

Karen H Miga (KH)

UC Santa Cruz Genomics Institute, University of California Santa Cruz, Santa Cruz, CA, USA.

Adam M Phillippy (AM)

Genome Informatics Section, Computational and Statistical Genomics Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, MD, USA.

Winston Timp (W)

Department of Molecular Biology and Genetics, Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD, USA.

Mario Ventura (M)

Department of Biology, University of Bari, Aldo Moro, Bari 70125, Italy.

Evan E Eichler (EE)

Department of Genome Sciences, University of Washington School of Medicine, Seattle, WA, USA.
Howard Hughes Medical Institute, University of Washington, Seattle, WA, USA.

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Classifications MeSH