A secreted ribonuclease effector from Verticillium dahliae localizes in the plant nucleus to modulate host immunity.
Verticillium dahliae
VdRTX1
host immunity
secreted ribonuclease
Journal
Molecular plant pathology
ISSN: 1364-3703
Titre abrégé: Mol Plant Pathol
Pays: England
ID NLM: 100954969
Informations de publication
Date de publication:
08 2022
08 2022
Historique:
revised:
21
01
2022
received:
03
08
2021
accepted:
11
03
2022
pubmed:
2
4
2022
medline:
15
7
2022
entrez:
1
4
2022
Statut:
ppublish
Résumé
The arms race between fungal pathogens and plant hosts involves recognition of fungal effectors to induce host immunity. Although various fungal effectors have been identified, the effector functions of ribonucleases are largely unknown. Herein, we identified a ribonuclease secreted by Verticillium dahliae (VdRTX1) that translocates into the plant nucleus to modulate immunity. The activity of VdRTX1 causes hypersensitive response (HR)-related cell death in Nicotiana benthamiana and cotton. VdRTX1 possesses a signal peptide but is unlikely to be an apoplastic effector because its nuclear localization in the plant is necessary for cell death induction. Knockout of VdRTX1 significantly enhanced V. dahliae virulence on tobacco while V. dahliae employs the known suppressor VdCBM1 to escape the immunity induced by VdRTX1. VdRTX1 homologs are widely distributed in fungi but transient expression of 24 homologs from other fungi did not yield cell death induction, suggesting that this function is specific to the VdRTX1 in V. dahliae. Expression of site-directed mutants of VdRTX1 in N. benthamiana leaves revealed conserved ligand-binding sites that are important for VdRTX1 function in inducing cell death. Thus, VdRTX1 functions as a unique HR-inducing effector in V. dahliae that contributes to the activation of plant immunity.
Identifiants
pubmed: 35363930
doi: 10.1111/mpp.13213
pmc: PMC9276946
doi:
Substances chimiques
Ribonucleases
EC 3.1.-
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1122-1140Informations de copyright
© 2022 The Authors. Molecular Plant Pathology published by British Society for Plant Pathology and John Wiley & Sons Ltd.
Références
Nature. 2011 Oct 05;478(7369):395-8
pubmed: 21976020
Nat Plants. 2015 Oct 05;1:15140
pubmed: 27251392
Nature. 2012 Apr 11;484(7393):186-94
pubmed: 22498624
J Mol Biol. 1993 Apr 5;230(3):979-96
pubmed: 8386773
Acta Crystallogr D Biol Crystallogr. 2010 Jul;66(Pt 7):843-9
pubmed: 20606265
Proc Natl Acad Sci U S A. 2009 Jun 23;106(25):10171-6
pubmed: 19520826
Plant Cell. 2010 Jan;22(1):260-72
pubmed: 20061552
Arch Virol. 2013 Dec;158(12):2621-3
pubmed: 23818047
Trends Immunol. 2014 Jul;35(7):345-51
pubmed: 24946686
Nucleic Acids Res. 2021 Jan 8;49(D1):D458-D460
pubmed: 33104802
Plant Cell. 2006 Dec;18(12):3721-44
pubmed: 17194768
PLoS Pathog. 2011 Jul;7(7):e1002137
pubmed: 21829347
New Phytol. 2019 Apr;222(2):1012-1029
pubmed: 30609067
Mol Microbiol. 2013 Jul;89(1):179-88
pubmed: 23692401
New Phytol. 2013 Jun;198(4):1001-1016
pubmed: 23594392
Gene. 1997 Oct 1;198(1-2):289-96
pubmed: 9370294
Phytopathology. 2014 Jun;104(6):564-74
pubmed: 24548214
Plant Cell. 1999 Dec;11(12):2291-301
pubmed: 10590159
Annu Rev Plant Biol. 2009;60:379-406
pubmed: 19400727
Genome Res. 2013 Aug;23(8):1271-82
pubmed: 23685541
Biol Chem. 2017 Jan 1;398(1):135-142
pubmed: 27472070
Nat Methods. 2011 Sep 29;8(10):785-6
pubmed: 21959131
Proteins. 2008 Jul;72(1):217-28
pubmed: 18214983
Virus Res. 2011 Jul;159(1):73-8
pubmed: 21571013
Front Plant Sci. 2019 Jun 26;10:822
pubmed: 31297126
Plant Cell. 2015 Jul;27(7):2057-72
pubmed: 26163574
New Phytol. 2018 Jan;217(2):756-770
pubmed: 29084346
Proc Natl Acad Sci U S A. 2014 Jun 3;111(22):8299-304
pubmed: 24847068
Nat Rev Microbiol. 2013 Nov;11(11):800-14
pubmed: 24129511
FEMS Microbiol Rev. 2007 Mar;31(2):212-37
pubmed: 17253975
New Phytol. 2015 Aug;207(3):735-47
pubmed: 25760731
Elife. 2018 May 14;7:
pubmed: 29757140
Proc Natl Acad Sci U S A. 2013 Jun 11;110(24):10010-5
pubmed: 23716655
Mol Plant Microbe Interact. 2013 Mar;26(3):278-86
pubmed: 23051172
New Phytol. 2017 Jul;215(1):368-381
pubmed: 28407259
Mol Plant Pathol. 2022 Aug;23(8):1122-1140
pubmed: 35363930
Mol Plant Microbe Interact. 2012 Jul;25(7):964-75
pubmed: 22414440
Mol Biol Evol. 2013 Dec;30(12):2725-9
pubmed: 24132122
Front Microbiol. 2016 Oct 28;7:1709
pubmed: 27840627
J Genet Genomics. 2013 Aug 20;40(8):421-31
pubmed: 23969251
Environ Microbiol. 2017 May;19(5):1914-1932
pubmed: 28205292
Plant Cell. 2014 Jun 24;26(6):2708-2723
pubmed: 24963057
Mol Plant Microbe Interact. 2018 Feb;31(2):260-273
pubmed: 29068240
Plant J. 2010 Feb;61(4):661-71
pubmed: 19947982
Nucleic Acids Res. 2015 Jul 1;43(W1):W174-81
pubmed: 25883148
Microbiol Mol Biol Rev. 2017 Mar 29;81(2):
pubmed: 28356329
Plant Cell. 2009 Sep;21(9):2928-47
pubmed: 19794118
Plant Cell. 2011 Jun;23(6):2440-55
pubmed: 21693696
J Mol Biol. 2001 Jan 19;305(3):567-80
pubmed: 11152613
Nat Rev Genet. 2010 Aug;11(8):539-48
pubmed: 20585331
Nucleic Acids Res. 2007 Jul;35(Web Server issue):W429-32
pubmed: 17483518
Proc Natl Acad Sci U S A. 1999 Jul 6;96(14):7956-61
pubmed: 10393929
Plant J. 2002 Sep;31(6):777-86
pubmed: 12220268
Annu Rev Phytopathol. 2009;47:39-62
pubmed: 19385730
J Biol Chem. 1992 Aug 25;267(24):16801-5
pubmed: 1512222
Gene. 2013 Oct 25;529(2):307-16
pubmed: 23891822
Methods. 2001 Dec;25(4):402-8
pubmed: 11846609
Biochim Biophys Acta. 1972 Apr 7;268(1):125-31
pubmed: 4336164
Front Plant Sci. 2021 Apr 09;12:641480
pubmed: 33897729
Annu Rev Plant Biol. 2015;66:513-45
pubmed: 25923844
Nature. 2006 Nov 16;444(7117):323-9
pubmed: 17108957
New Phytol. 2018 Jan;217(1):320-331
pubmed: 28895153
PLoS Pathog. 2019 Sep 3;15(9):e1007951
pubmed: 31479498
Curr Opin Plant Biol. 2014 Aug;20:96-103
pubmed: 24879450
PLoS Pathog. 2019 Mar 11;15(3):e1007620
pubmed: 30856238
Science. 2009 Jan 2;323(5910):95-101
pubmed: 19095898
Mol Biol Evol. 2016 Jul;33(7):1870-4
pubmed: 27004904
Nucleic Acids Res. 2007 Jul;35(Web Server issue):W585-7
pubmed: 17517783
Nat Commun. 2014 Aug 26;5:4686
pubmed: 25156390
Curr Opin Immunol. 2008 Feb;20(1):10-6
pubmed: 18206360
Science. 2010 Aug 20;329(5994):953-5
pubmed: 20724636
Toxicon. 1999 Nov;37(11):1549-63
pubmed: 10482390
Proc Natl Acad Sci U S A. 2014 Nov 25;111(47):16955-60
pubmed: 25368167
J Gen Microbiol. 1992 Jul;138(7):1429-35
pubmed: 1512575
Mol Plant Pathol. 2020 May;21(5):667-685
pubmed: 32314529
Eur J Biochem. 1997 Jul 1;247(1):1-11
pubmed: 9249002
Mol Plant Microbe Interact. 2018 Jan;31(1):34-45
pubmed: 29144205
Nucleic Acids Res. 1997 Dec 15;25(24):4876-82
pubmed: 9396791
Proc Natl Acad Sci U S A. 2009 Jun 23;106(25):10359-64
pubmed: 19520828
Protein Sci. 1999 Apr;8(4):722-30
pubmed: 10211818
Toxins (Basel). 2017 Feb 21;9(2):
pubmed: 28230789