A new approach to zip-lignin: 3,4-dihydroxybenzoate is compatible with lignification.
Populus alba × grandidentata
acylated monolignols
biomass feedstock engineering
lignin valorization
poplar trees
zip-lignin
Journal
The New phytologist
ISSN: 1469-8137
Titre abrégé: New Phytol
Pays: England
ID NLM: 9882884
Informations de publication
Date de publication:
07 2022
07 2022
Historique:
received:
02
12
2021
accepted:
17
03
2022
pubmed:
5
4
2022
medline:
7
6
2022
entrez:
4
4
2022
Statut:
ppublish
Résumé
Renewed interests in the development of bioenergy, biochemicals, and biomaterials have elicited new strategies for engineering the lignin of biomass feedstock plants. This study shows, for the first time, that 3,4-dihydroxybenzoate (DHB) is compatible with the radical coupling reactions that assemble polymeric lignin in plants. We introduced a bacterial 3-dehydroshikimate dehydratase into hybrid poplar (Populus alba × grandidentata) to divert carbon flux away from the shikimate pathway, which lies upstream of lignin biosynthesis. Transgenic poplar wood had up to 33% less lignin with p-hydroxyphenyl units comprising as much as 10% of the lignin. Mild alkaline hydrolysis of transgenic wood released fewer ester-linked p-hydroxybenzoate groups than control trees, and revealed the novel incorporation of cell-wall-bound DHB, as well as glycosides of 3,4-dihydroxybenzoic acid (DHBA). Two-dimensional nuclear magnetic resonance (2D-NMR) analysis uncovered DHBA-derived benzodioxane structures suggesting that DHB moieties were integrated into the lignin polymer backbone. In addition, up to 40% more glucose was released from transgenic wood following ionic liquid pretreatment and enzymatic hydrolysis. This work highlights the potential of diverting carbon flux from the shikimate pathway for lignin engineering and describes a new type of 'zip-lignin' derived from the incorporation of DHB into poplar lignin.
Identifiants
pubmed: 35377486
doi: 10.1111/nph.18136
pmc: PMC9325543
doi:
Substances chimiques
3,4-dihydroxybenzoate
0
Hydroxybenzoates
0
Lignin
9005-53-2
Types de publication
Journal Article
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
234-246Informations de copyright
© 2022 The Authors New Phytologist © 2022 New Phytologist Foundation.
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