Force-dependent intercellular adhesion strengthening underlies asymmetric adherens junction contraction.
RhoA, optogenetics, adhesion clutch, junction mechanics
Journal
Current biology : CB
ISSN: 1879-0445
Titre abrégé: Curr Biol
Pays: England
ID NLM: 9107782
Informations de publication
Date de publication:
09 05 2022
09 05 2022
Historique:
received:
07
04
2021
revised:
04
01
2022
accepted:
08
03
2022
pubmed:
6
4
2022
medline:
14
5
2022
entrez:
5
4
2022
Statut:
ppublish
Résumé
Tissue morphogenesis arises from the culmination of changes in cell-cell junction length. Mechanochemical signaling in the form of RhoA underlies these ratcheted contractions, which occur asymmetrically. The underlying mechanisms of asymmetry remain unknown. We use optogenetically controlled RhoA in model epithelia together with biophysical modeling to uncover the mechanism lending to asymmetric vertex motion. Using optogenetic and pharmacological approaches, we find that both local and global RhoA activation can drive asymmetric junction contraction in the absence of tissue-scale patterning. We find that standard vertex models with homogeneous junction properties are insufficient to recapitulate the observed junction dynamics. Furthermore, these experiments reveal a local coupling of RhoA activation with E-cadherin accumulation. This motivates a coupling of RhoA-mediated increases in tension and E-cadherin-mediated adhesion strengthening. We then demonstrate that incorporating this force-sensitive adhesion strengthening into a continuum model is successful in capturing the observed junction dynamics. Thus, we find that a force-dependent intercellular "clutch" at tricellular vertices stabilizes vertex motion under increasing tension and is sufficient to generate asymmetries in junction contraction.
Identifiants
pubmed: 35381185
pii: S0960-9822(22)00419-5
doi: 10.1016/j.cub.2022.03.024
pmc: PMC9123775
mid: NIHMS1798963
pii:
doi:
Substances chimiques
Cadherins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
1986-2000.e5Subventions
Organisme : NIGMS NIH HHS
ID : R01 GM104032
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM143042
Pays : United States
Organisme : NIGMS NIH HHS
ID : T32 GM007183
Pays : United States
Organisme : Howard Hughes Medical Institute
Pays : United States
Commentaires et corrections
Type : ErratumIn
Informations de copyright
Copyright © 2022 Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of interests The authors declare no competing interests.
Références
Dev Cell. 2019 Jun 17;49(6):894-906.e12
pubmed: 31105010
Annu Rev Cell Dev Biol. 2011;27:157-84
pubmed: 21740231
Science. 2014 Oct 31;346(6209):1254211
pubmed: 25359979
Nat Cell Biol. 2011 May;13(5):529-40
pubmed: 21516109
Nature. 2010 Dec 23;468(7327):1110-4
pubmed: 21068726
Nat Methods. 2019 Sep;16(9):894-901
pubmed: 31384043
Curr Biol. 2007 Dec 18;17(24):2095-104
pubmed: 18082406
Philos Trans R Soc Lond B Biol Sci. 2017 May 19;372(1720):
pubmed: 28348254
Cell Rep. 2017 Mar 21;18(12):2854-2867
pubmed: 28329679
Phys Rev X. 2020 Jan-Mar;10(1):
pubmed: 33767909
Nature. 2008 Jun 5;453(7196):751-6
pubmed: 18480755
Nature. 2015 Aug 20;524(7565):351-5
pubmed: 26214737
J Cell Biol. 2016 Apr 25;213(2):243-60
pubmed: 27114502
Nat Methods. 2012 Jul;9(7):671-5
pubmed: 22930834
Curr Protoc Cell Biol. 2020 Mar;86(1):e102
pubmed: 32031760
Dev Cell. 2013 Jul 29;26(2):162-75
pubmed: 23871590
Cell. 2009 Jun 26;137(7):1331-42
pubmed: 19563762
J Cell Biol. 2013 Apr 1;201(1):131-43
pubmed: 23547031
ACS Chem Biol. 2011 May 20;6(5):452-65
pubmed: 21241068
Biophys J. 2014 Jun 3;106(11):2291-304
pubmed: 24896108
J Cell Biol. 2016 Aug 29;214(5):603-17
pubmed: 27551058
J Cell Biol. 2016 Jun 20;213(6):641-9
pubmed: 27298323
Nat Commun. 2017 Sep 7;8(1):476
pubmed: 28883443
Dev Cell. 2018 Nov 19;47(4):439-452.e6
pubmed: 30318244
Curr Biol. 2008 Jan 8;18(1):30-6
pubmed: 18158243
Nat Commun. 2017 Jun 12;8:15817
pubmed: 28604737
Trends Cell Biol. 2007 Jan;17(1):36-43
pubmed: 17126549
Annu Rev Cell Dev Biol. 2010;26:315-33
pubmed: 19575647
Nat Commun. 2014 Jun 02;5:3941
pubmed: 24887573
Elife. 2021 May 25;10:
pubmed: 34032216
Nat Cell Biol. 2016 Mar;18(3):261-70
pubmed: 26780298
Nat Methods. 2012 Mar 04;9(4):379-84
pubmed: 22388287
Dev Cell. 2020 Jan 27;52(2):152-166.e5
pubmed: 31883774
Curr Biol. 2019 Feb 18;29(4):578-591.e5
pubmed: 30744966
Biophys J. 2019 Nov 5;117(9):1739-1750
pubmed: 31635790
Nat Commun. 2017 Oct 5;8(1):790
pubmed: 28983097
Curr Biol. 2010 Jul 13;20(13):1145-53
pubmed: 20541412
Phys Biol. 2011 Aug;8(4):045005
pubmed: 21750365
Elife. 2018 Jul 09;7:
pubmed: 29985789
F1000Res. 2019 Jul 10;8:
pubmed: 31327995
Nat Cell Biol. 2013 Aug;15(8):926-36
pubmed: 23831726