Anti-adipogenic β-sitosterol and lupeol from Moringa oleifera suppress adipocyte differentiation through regulation of cell cycle progression.
Moringa oleifera
adipogenesis
cell cycle analysis
lupeol
obesity
β-Sitosterol
Journal
Journal of food biochemistry
ISSN: 1745-4514
Titre abrégé: J Food Biochem
Pays: United States
ID NLM: 7706045
Informations de publication
Date de publication:
08 2022
08 2022
Historique:
revised:
22
02
2022
received:
02
12
2021
accepted:
22
03
2022
pubmed:
12
4
2022
medline:
11
8
2022
entrez:
11
4
2022
Statut:
ppublish
Résumé
Triterpenes and phytosterols enriched herbal formulations are known for glucose regulation and lipid metabolism. In this study, triterpenes and phytosterols from Moringa oleifera stem bark have been tested for their role in adipocyte differentiation. Chromatographic analysis revealed a wide range of phenolics, highlighting the presence of flavonoids (kaempferol, quercetin, and rutin), terpenoids (lupeol), and phytosterol (stigmasterol, β-sitosterol). Lupeol and β-sitosterol reduced cell viability in a dose-dependent manner showcasing increased G
Substances chimiques
Pentacyclic Triterpenes
0
Sitosterols
0
Triterpenes
0
gamma-sitosterol
5LI01C78DD
Glucose
IY9XDZ35W2
lupeol
O268W13H3O
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e14170Informations de copyright
© 2022 Wiley Periodicals LLC.
Références
Antignac, E., Nohynek, G. J., Re, T., Clouzeau, J., & Toutain, H. (2011). Safety of botanical ingredients in personal care products/cosmetics. Food and Chemical Toxicology, 49, 324-341. https://doi.org/10.1016/j.fct.2010.11.022
Anwar, F., Latif, S., Ashraf, M., & Gilani, A. H. (2007). Moringa oleifera: A food plant with multiple medicinal uses. Pharmacological Research, 21, 17-25. https://doi.org/10.1002/ptr.2023
Aronne, L. J., & Isoldi, K. K. (2007). Overweight and obesity: Key components of cardiometabolic risk. Clinical Cornerstone, 8, 29-37. https://doi.org/10.1016/S1098-3597(07)80026-3
Asha, R., Gayathri Devi, V., & Abraham, A. (2016). Lupeol, a pentacyclic triterpenoid isolated from Vernonia cinerea attenuate selenite induced cataract formation in Sprague Dawley rat pups. Chemico-Biological Interactions, 245, 20-29. https://doi.org/10.1016/j.cbi.2015.12.002
de Melo, C. L., Queiroz, M. G. R., Arruda Filho, A. C. V., Rodrigues, A. M., de Sousa, D. F., Almeida, J. G. L., Pessoa, O. D. L., Silveira, E. R., Menezes, D. B., Melo, T. S., Santos, F. A., & Rao, V. S. (2009). Betulinic acid, a natural Pentacyclic Triterpenoid, prevents abdominal fat accumulation in mice fed a high-fat diet. Journal of Agricultural and Food Chemistry, 57, 8776-8781. https://doi.org/10.1021/jf900768w
de Melo, C. L., Queiroz, M. G. R., Fonseca, S. G. C., Bizerra, A. M. C., Lemos, T. L. G., Melo, T. S., Santos, F. A., & Rao, V. S. (2010). Oleanolic acid, a natural triterpenoid improves blood glucose tolerance in normal mice and ameliorates visceral obesity in mice fed a high-fat diet. Chemico-Biological Interactions, 185, 59-65. https://doi.org/10.1016/j.cbi.2010.02.028
Heal, D. J., Gosden, J., & Smith, S. L. (2009). Regulatory challenges for new drugs to treat obesity and comorbid metabolic disorders. British Journal of Clinical Pharmacology, 68, 861-874. https://doi.org/10.1111/j.1365-2125.2009.03549.x
Hsu, B.-Y., Pan, S.-Y., Wu, L.-Y., Ho, C.-T., & Hwang, L. S. (2018). Hypoglycemic activity of Chenopodium formosanum Koidz. Components using a glucose uptake assay with 3T3-L1 adipocytes. Food Bioscience, 24, 9-16. https://doi.org/10.1016/j.fbio.2018.05.001
Jang, M., Choi, H. Y., & Kim, G. H. (2019). Inhibitory effects of Orostachys malacophyllus var. iwarenge extracts on reactive oxygen species production and lipid accumulation during 3T3-L1 adipocyte differentiation. Food Science and Biotechnology, 28, 227-236. https://doi.org/10.1007/s10068-018-0426-x
Kanda, K., Nishi, K., Kadota, A., Nishimoto, S., Liu, M.-C., & Sugahara, T. (2012). Nobiletin suppresses adipocyte differentiation of 3T3-L1 cells by an insulin and IBMX mixture induction. Biochimica et Biophysica Acta-General Subjects, 1820, 461-468. https://doi.org/10.1016/j.bbagen.2011.11.015
Karthick, V., Panda, S., Kumar, V. G., Kumar, D., Shrestha, L. K., Ariga, K., Vasanth, K., Chinnathambi, S., Dhas, T. S., & Suganya, K. S. U. (2019). Quercetin loaded PLGA microspheres induce apoptosis in breast cancer cells. Applied Surface Science, 487, 211-217. https://doi.org/10.1016/j.apsusc.2019.05.047
Khan, N., Afaq, F., & Mukhtar, H. (2008). Cancer chemoprevention through dietary antioxidants: Progress and promise. Antioxidants & Redox Signaling, 10, 475-510. https://doi.org/10.1089/ars.2007.1740
Kowalska, K., Olejnik, A., Rychlik, J., & Grajek, W. (2015). Cranberries (Oxycoccus quadripetalus) inhibit lipid metabolism and modulate leptin and adiponectin secretion in 3T3-L1 adipocytes. Food Chemistry, 185, 383-388. https://doi.org/10.1016/j.foodchem.2015.03.152
Li, K. K., Peng, J. M., Zhu, W., Cheng, B. H., & Li, C. M. (2017). Gallocatechin gallate (GCG) inhibits 3T3-L1 differentiation and lipopolysaccharide induced inflammation through MAPK and NF-κB signaling. Journal of Functional Foods, 30, 159-167. https://doi.org/10.1016/j.jff.2017.01.016
Lorent, J., Le Duff, C. S., Quetin-Leclercq, J., & Mingeot-Leclercq, M.-P. (2013). Induction of highly curved structures in relation to membrane Permeabilization and budding by the Triterpenoid Saponins, α- and δ-Hederin. The Journal of Biological Chemistry, 288, 14000-14017. https://doi.org/10.1074/jbc.M112.407635
Minakshi, G. C., Vasanth, K., Priya, T., Ilango, K., Agrawal, A., & Dubey, G. P. (2015). Inhibitory potential of Terminalia chebula by hydrogen peroxide induced oxidative stress in THP-1 cell line. International Journal of Botany, 11, 21-26. https://doi.org/10.3923/ijb.2015.21.26
Mochizuki, K., Yamada, M., Inoue, T., Hariya, N., Kubota, T., & Goda, T. (2020). Bromodomain-containing protein 4 regulates a cascade of lipid-accumulation-related genes at the transcriptional level in the 3T3-L1 white adipocyte-like cell line. European Journal of Pharmacology, 883, 173351. https://doi.org/10.1016/j.ejphar.2020.173351
Muniyappa, R., & Gubbi, S. (2020). COVID-19 pandemic, coronaviruses, and diabetes mellitus. American Journal of Physiology-Endocrinology and Metabolism, 318, E736-E741. https://doi.org/10.1152/AJPENDO.00124.2020/ASSET/IMAGES/LARGE/ZH10052083420002.JPEG
Park, Y. M., Kim, J. I., Seo, D. H., Seo, J. H., Lim, J.-H., Lee, J. E., Choi, J.-Y., & Seo, E.-W. (2018). Repressive effects of red bean, Phaseolus angularis, extracts on obesity of mouse induced with high-fat diet via downregulation of adipocyte differentiation and modulating lipid metabolism. Food Science and Biotechnology, 27, 1811-1821. https://doi.org/10.1007/s10068-018-0421-2
Radika, M. K., Viswanathan, P., & Anuradha, C. V. (2013). Nitric oxide mediates the insulin sensitizing effects of β-sitosterol in high fat diet-fed rats. Nitric Oxide, 32, 43-53. https://doi.org/10.1016/j.niox.2013.04.007
Rosen, E. D., & Spiegelman, B. M. (2006). Adipocytes as regulators of energy balance and glucose homeostasis. Nature, 444, 847-853. https://doi.org/10.1038/nature05483
Salehi, B., Fokou, P. V. T., Yamthe, L. R. T., Tali, B. T., Adetunji, C. O., Rahavian, A., Mudau, F. N., Martorell, M., Setzer, W. N., Rodrigues, C. F., Martins, N., Cho, W. C., & Sharifi-Rad, J. (2019). Phytochemicals in prostate cancer: From bioactive molecules to upcoming therapeutic agents. Nutrients, 11, 1483. https://doi.org/10.3390/nu11071483
Sánchez-Burgos, J. A., Ramírez-Mares, M. V., Gallegos-Infante, J. A., González-Laredo, R. F., Moreno-Jiménez, M. R., Cháirez-Ramírez, M. H., Medina-Torres, L., & Rocha-Guzmán, N. E. (2015). Isolation of lupeol from white oak leaves and its anti-inflammatory activity. Industrial Crops and Products, 77, 827-832. https://doi.org/10.1016/j.indcrop.2015.09.056
Sudhahar, V., Ashok Kumar, S., Varalakshmi, P., & Sujatha, V. (2008). Protective effect of lupeol and lupeol linoleate in hypercholesterolemia associated renal damage. Molecular and Cellular Biochemistry, 317, 11-20. https://doi.org/10.1007/s11010-008-9786-5
Sudhahar, V., Kumar, S. A., & Varalakshmi, P. (2006). Role of lupeol and lupeol linoleate on lipemic-oxidative stress in experimental hypercholesterolemia. Life Sciences, 78, 1329-1335. https://doi.org/10.1016/j.lfs.2005.07.011
Sung, H.-Y., Kang, S.-W., Kim, J.-L., Li, J., Lee, E.-S., Gong, J.-H., Han, S. J., & Kang, Y.-H. (2010). Oleanolic acid reduces markers of differentiation in 3T3-L1 adipocytes. Nutrition Research, 30, 831-839. https://doi.org/10.1016/j.nutres.2010.10.001
Vasanth, K., Ilango, K., MohanKumar, R., Agrawal, A., & Dubey, G. P. (2014). Anticancer activity of Moringa oleifera mediated silver nanoparticles on human cervical carcinoma cells by apoptosis induction. Colloids Surfaces B Biointerfaces, 117, 354-359. https://doi.org/10.1016/j.colsurfb.2014.02.052
Vasanth, K., Minakshi, G. C., Ilango, K., Kumar, R. M., Agrawal, A., & Dubey, G. P. (2015). Moringa oleifera attenuates the release of pro-inflammatory cytokines in lipopolysaccharide stimulated human monocytic cell line. Industrial Crops and Products, 77, 44-50. https://doi.org/10.1016/j.indcrop.2015.08.013
Verma, A. R., Vijayakumar, M., Mathela, C. S., & Rao, C. V. (2009). In vitro and in vivo antioxidant properties of different fractions of Moringa oleifera leaves. Food and Chemical Toxicology, 47, 2196-2201. https://doi.org/10.1016/j.fct.2009.06.005
Wang, L., Xu, M. L., Rasmussen, S. K., & Wang, M.-H. (2011). Vomifoliol 9-O-α-arabinofuranosyl (1→6)-β-d-glucopyranoside from the leaves of Diospyros Kaki stimulates the glucose uptake in HepG2 and 3T3-L1 cells. Carbohydrate Research, 346, 1212-1216. https://doi.org/10.1016/j.carres.2011.04.021
Wang, S., Wu, S., & Liu, S. (2019). Integration of (+)-catechin and β-sitosterol to achieve excellent radical-scavenging activity in emulsions. Food Chemistry, 272, 596-603. https://doi.org/10.1016/j.foodchem.2018.08.098
Wang, X., Huang, W., Lei, L., Liu, Y., Ma, K. Y., Li, Y. M., Wang, L., Huang, Y., & Chen, Z.-Y. (2015). Blockage of hydroxyl group partially abolishes the cholesterol-lowering activity of β-sitosterol. Journal of Functional Foods, 12, 199-207. https://doi.org/10.1016/j.jff.2014.11.019
Xu, G., Sun, J., Liang, Y., Yang, C., & Chen, Z.-Y. (2011). Interaction of fatty acids with oxidation of cholesterol and β-sitosterol. Food Chemistry, 124, 162-170. https://doi.org/10.1016/j.foodchem.2010.06.003
Xu, M., Xiao, S., Sun, Y., Ou-yang, Y., Guan, C., & Zheng, X. (2006). A Preadipocyte differentiation assay as a method for screening potential anti-type II diabetes drugs from herbal extracts. Planta Medica, 72, 14-19. https://doi.org/10.1055/s-2005-916215
Yu, S. C., Chen, T. C., Hou, Y. T., Wan, L., Tsai, F. J., & Tsai, Y. (2018). β-Sitosterol-2-hydroxypropyl-β-cyclodextrin inclusion complex: Characterization and inhibitory effect on adipogenesis in 3T3-L1 pre-adipocytes. Steroids, 140, 196-201. https://doi.org/10.1016/j.steroids.2018.08.010
Zebisch, K., Voigt, V., Wabitsch, M., & Brandsch, M. (2012). Protocol for effective differentiation of 3T3-L1 cells to adipocytes. Analytical Biochemistry, 425, 88-90. https://doi.org/10.1016/j.ab.2012.03.005
Zhang, T., Qin, X., Cao, Y., Zhang, J., & Zhao, J. (2020). Sea buckthorn (Hippophae rhamnoides L.) oil enhances proliferation, adipocytes differentiation and insulin sensitivity in 3T3-L1 cells. Food Science and Biotechnology, 29, 1511-1518. https://doi.org/10.1007/s10068-020-00817-4
Zou, C., Wang, Y., & Shen, Z. (2005). 2-NBDG as a fluorescent indicator for direct glucose uptake measurement. Journal of Biochemical and Biophysical Methods, 64, 207-215. https://doi.org/10.1016/j.jbbm.2005.08.001