A dual-function phage regulator controls the response of cohabiting phage elements via regulation of the bacterial SOS response.
CP: Microbiology
KilAC
Listeria monocytogenes
SOS response
ant
bacteriophage
lysogeny
pathogen
phage
Journal
Cell reports
ISSN: 2211-1247
Titre abrégé: Cell Rep
Pays: United States
ID NLM: 101573691
Informations de publication
Date de publication:
19 04 2022
19 04 2022
Historique:
received:
08
06
2021
revised:
09
02
2022
accepted:
30
03
2022
entrez:
20
4
2022
pubmed:
21
4
2022
medline:
23
4
2022
Statut:
ppublish
Résumé
Listeria monocytogenes strain 10403S harbors two phage elements in its chromosome; one produces infective virions and the other tailocins. It was previously demonstrated that induction of the two elements is coordinated, as they are regulated by the same anti-repressor. In this study, we identified AriS as another phage regulator that controls the two elements, bearing the capacity to inhibit their lytic induction under SOS conditions. AriS is a two-domain protein that possesses two distinct activities, one regulating the genes of its encoding phage and the other downregulating the bacterial SOS response. While the first activity associates with the AriS N-terminal AntA/AntB domain, the second associates with its C-terminal ANT/KilAC domain. The ANT/KilAC domain is conserved in many AriS-like proteins of listerial and non-listerial prophages, suggesting that temperate phages acquired such dual-function regulators to align their response with the other phage elements that cohabit the genome.
Identifiants
pubmed: 35443160
pii: S2211-1247(22)00484-3
doi: 10.1016/j.celrep.2022.110723
pmc: PMC9043618
pii:
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
110723Informations de copyright
Copyright © 2022 The Authors. Published by Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of interests The authors declare no competing interests.
Références
Evol Appl. 2015 Apr;8(4):346-51
pubmed: 25926879
Cell Host Microbe. 2020 Apr 8;27(4):629-641.e4
pubmed: 32101705
J Mol Biol. 1989 May 5;207(1):135-49
pubmed: 2661831
Annu Rev Microbiol. 1999;53:217-44
pubmed: 10547691
J Bacteriol. 1984 May;158(2):551-61
pubmed: 6327610
Proc Natl Acad Sci U S A. 2016 May 3;113(18):E2480-8
pubmed: 27099293
Nucleic Acids Res. 2015 Sep 3;43(15):7315-29
pubmed: 26138485
Mol Cell. 2009 Oct 9;36(1):121-30
pubmed: 19818715
J Bacteriol. 2007 Sep;189(17):6333-8
pubmed: 17586637
Curr Opin Microbiol. 2017 Aug;38:81-87
pubmed: 28544996
J Bacteriol. 2016 Sep 22;198(20):2784-93
pubmed: 27457717
Proc Natl Acad Sci U S A. 2011 Dec 6;108(49):19484-91
pubmed: 22114192
Nat Rev Microbiol. 2009 Sep;7(9):623-8
pubmed: 19648949
Mol Cell. 2005 Jan 21;17(2):291-300
pubmed: 15664197
FEMS Microbiol Lett. 2016 Mar;363(5):fnw015
pubmed: 26825679
Annu Rev Genet. 2000;34:479-497
pubmed: 11092836
Genome Biol. 2002;3(3):RESEARCH0012
pubmed: 11897024
Cell. 2012 Aug 17;150(4):792-802
pubmed: 22901809
Cell Rep. 2020 Jul 28;32(4):107956
pubmed: 32726621
J Cell Biol. 2002 Aug 5;158(3):409-14
pubmed: 12163465
Microbiology (Reading). 1995 Oct;141 ( Pt 10):2577-84
pubmed: 7582018
Virology. 2006 Aug 1;351(2):360-7
pubmed: 16713610
Appl Environ Microbiol. 2017 Mar 2;83(6):
pubmed: 28039138
Proteomics. 2011 Nov;11(21):4155-65
pubmed: 21956863
Biochimie. 1991 Apr;73(4):411-21
pubmed: 1911941
Nat Commun. 2019 Nov 21;10(1):5288
pubmed: 31754112
Cell Host Microbe. 2020 Apr 8;27(4):493-495
pubmed: 32272072
J Bacteriol. 1988 May;170(5):2163-73
pubmed: 2834329
Annu Rev Genet. 2005;39:409-29
pubmed: 16285866
Structure. 2019 Jul 2;27(7):1094-1102.e4
pubmed: 31056420
Protein Expr Purif. 2019 Feb;154:104-111
pubmed: 30326268
Nat Rev Microbiol. 2015 Oct;13(10):641-50
pubmed: 26373372
Cold Spring Harb Perspect Biol. 2013 Nov 01;5(11):a012674
pubmed: 24097899
Virology. 2015 May;479-480:310-30
pubmed: 25742714
FEBS Lett. 1993 Nov 15;334(2):165-9
pubmed: 8224242
Mol Microbiol. 2003 Jul;49(2):277-300
pubmed: 12886937
J Virol. 2001 Dec;75(23):11720-34
pubmed: 11689653
J Mol Biol. 1990 Oct 5;215(3):403-10
pubmed: 2231712
Mol Microbiol. 2000 Jan;35(2):324-40
pubmed: 10652093