Co-immunoprecipitation Assays.

Immunoprecipitation Protein Binding Proteins / metabolism
Protein–protein interaction Signal transduction VEGF p130Cas

Journal

Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969

Informations de publication

Date de publication:
2022
Historique:
entrez: 22 4 2022
pubmed: 23 4 2022
medline: 27 4 2022
Statut: ppublish

Résumé

Co-immunoprecipitation is a well-established technique for determining whether two proteins interact. It is based on the principle that by pulling down one protein, you will also obtain any other proteins that exist in a complex with that protein. It is a relatively simple technique that does not require expensive reagents or materials. It is however, not without its limitations and some of these will be discussed here along with a step-by-step guide to performing and analyzing co-immunoprecipitation experiments.

Identifiants

DOI: 10.1007/978-1-0716-2217-9_8 PMID: 35451753
pubmed: 35451753
doi: 10.1007/978-1-0716-2217-9_8
doi:

Substances chimiques

Proteins 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

125-132

Informations de copyright

© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Références

Miura K (2018) An overview of current methods to confirm protein-protein interactions. Protein Pept Lett 25(8):728–733. https://doi.org/10.2174/0929866525666180821122240
doi: 10.2174/0929866525666180821122240 pubmed: 30129399 pmcid: 6204658
Evans IM, Kennedy SA, Paliashvili K, Santra T, Yamaji M, Lovering RC, Britton G, Frankel P, Kolch W, Zachary IC (2017) Vascular endothelial growth factor (VEGF) promotes assembly of the p130Cas interactome to drive endothelial chemotactic signaling and angiogenesis. Mol Cell Proteomics 16(2):168–180. https://doi.org/10.1074/mcp.M116.064428
doi: 10.1074/mcp.M116.064428 pubmed: 28007913
Margineanu A, Chan JJ, Kelly DJ, Warren SC, Flatters D, Kumar S, Katan M, Dunsby CW, French PM (2016) Screening for protein-protein interactions using Forster resonance energy transfer (FRET) and fluorescence lifetime imaging microscopy (FLIM). Sci Rep 6:28186. https://doi.org/10.1038/srep28186
doi: 10.1038/srep28186 pubmed: 27339025 pmcid: 4919659

Auteurs

Ian M Evans (IM)

Breast Cancer Research, Cancer Stem Cell Team, Institute of Cancer Research, Chester Beatty Laboratories, London, UK. ian.evans@icr.ac.uk.

Ketevan Paliashvili (K)

Centre for Precision Healthcare, UCL Division of Medicine, University College London, London, UK. k.paliashvili@ucl.ac.uk.

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Classifications MeSH

questionsmedicales.fr › Techniques d'investigation › Techniques immunologiques › Immunoprécipitation › Co-immunoprecipitation Assays.
questionsmedicales.fr › Métabolisme › Liaison aux protéines › Co-immunoprecipitation Assays.
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Co-immunoprecipitation Assays.