Ligand fishing based on tubular microchannel modified with monoamine oxidase B for screening of the enzyme's inhibitors from Crocus sativus and Edgeworthia gardneri.
bioactive compounds
ligand fishing
modified microchannel
monoamine oxidase B
screening
Journal
Journal of separation science
ISSN: 1615-9314
Titre abrégé: J Sep Sci
Pays: Germany
ID NLM: 101088554
Informations de publication
Date de publication:
Jul 2022
Jul 2022
Historique:
revised:
24
03
2022
received:
20
01
2022
accepted:
17
04
2022
pubmed:
24
4
2022
medline:
30
7
2022
entrez:
23
4
2022
Statut:
ppublish
Résumé
A novel strategy of performing ligand fishing with enzyme-modified open tubular microchannel was proposed for screening bioactive components present in medicinal plants. Monoamine oxidase B was immobilized onto the surface of the microchannel for the first time to specifically extract its ligands when the plant's extracts solution flows through the channel. The thermal and the storage stability of immobilized monoamine oxidase B were significantly enhanced after immobilization. Crocin I and Ⅱ were extracted from Crocus sativus, and tiliroside was extracted from Edgeworthia gardneri. All the three compounds were inhibitors of the enzyme with the half-maximal inhibitory concentration values of 26.70 ± 0.91, 19.88 ± 2.78, and 15.65 ± 0.85 μM, respectively. The enzyme inhibition kinetics and molecular docking were investigated. This is the first report on the inhibitory effects of tiliroside and crocin Ⅱ. The novel ligand fishing method proposed in this work possesses advantages of rapidness, high efficiency, and tiny sample consumption compared to routine ligand fishing, with promising potential for screening active natural products in complex mixtures.
Identifiants
pubmed: 35461190
doi: 10.1002/jssc.202200057
doi:
Substances chimiques
Ligands
0
Monoamine Oxidase
EC 1.4.3.4
Plant Extracts
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
2394-2405Subventions
Organisme : Chinese Academy of Sciences
ID : KFJ-BRP-008
Organisme : the US National Institutes of Health
ID : 1U54MD015929
Informations de copyright
© 2022 Wiley-VCH GmbH.
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